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Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500
Using an Illumina platform, we sequenced the complete mitochondrial genome of Lanius sphenocercus sphenocercus to an average coverage of 1669.5×. We performed a de novo assembly using SOAPdenovo2 and obtained the total mitogenome with 16,833 bp in length. Most PCGs begin with the typical ATG start c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799910/ https://www.ncbi.nlm.nih.gov/pubmed/33473475 http://dx.doi.org/10.1080/23802359.2016.1167640 |
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author | Yang, Mei-Xia Wang, Qing-Xiong Xiao, Hong Yang, Chao |
author_facet | Yang, Mei-Xia Wang, Qing-Xiong Xiao, Hong Yang, Chao |
author_sort | Yang, Mei-Xia |
collection | PubMed |
description | Using an Illumina platform, we sequenced the complete mitochondrial genome of Lanius sphenocercus sphenocercus to an average coverage of 1669.5×. We performed a de novo assembly using SOAPdenovo2 and obtained the total mitogenome with 16,833 bp in length. Most PCGs begin with the typical ATG start codon with the exception of COI gene, which use GTG as the initiation codon. Stop codons AGG, TAG, TAA, and AGA are present in the PCGs; exceptions are COII, COIII and ND4, which possess incomplete termination codons (T). But, the function of COII with incomplete stop codon T should be further investigated. The phylogeny revealed that genetic distance of Laniidae and Corvidae was closer than other species. Compared to other three shrike species, L. s. sphenocercus occupy a separate status in the genus Lanius. |
format | Online Article Text |
id | pubmed-7799910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-77999102021-01-19 Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500 Yang, Mei-Xia Wang, Qing-Xiong Xiao, Hong Yang, Chao Mitochondrial DNA B Resour Mitogenome Announcement Using an Illumina platform, we sequenced the complete mitochondrial genome of Lanius sphenocercus sphenocercus to an average coverage of 1669.5×. We performed a de novo assembly using SOAPdenovo2 and obtained the total mitogenome with 16,833 bp in length. Most PCGs begin with the typical ATG start codon with the exception of COI gene, which use GTG as the initiation codon. Stop codons AGG, TAG, TAA, and AGA are present in the PCGs; exceptions are COII, COIII and ND4, which possess incomplete termination codons (T). But, the function of COII with incomplete stop codon T should be further investigated. The phylogeny revealed that genetic distance of Laniidae and Corvidae was closer than other species. Compared to other three shrike species, L. s. sphenocercus occupy a separate status in the genus Lanius. Taylor & Francis 2016-04-19 /pmc/articles/PMC7799910/ /pubmed/33473475 http://dx.doi.org/10.1080/23802359.2016.1167640 Text en © 2016 The Author(s). Published by Taylor & Francis. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Mitogenome Announcement Yang, Mei-Xia Wang, Qing-Xiong Xiao, Hong Yang, Chao Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500 |
title | Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500 |
title_full | Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500 |
title_fullStr | Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500 |
title_full_unstemmed | Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500 |
title_short | Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500 |
title_sort | sequencing complete mitochondrial genome of lanius sphenocercus sphenocercus (passeriformes: laniidae) using illunima hiseq 2500 |
topic | Mitogenome Announcement |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799910/ https://www.ncbi.nlm.nih.gov/pubmed/33473475 http://dx.doi.org/10.1080/23802359.2016.1167640 |
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