Illumina next-generation sequencing reveals the mitochondrial genome of Ducetia japonica (Orthoptera: Tettigoniidae)

Using Illumina next-generation sequencing (NGS), the complete mitochondrial genome (mitogenome) of Ducetia japonica was sequenced in the present study. The mitogenome of D. japonica (Genbank accession no. KU885974) is 16,276 bp in size, had the typical invertebrate mitochondrial gene arrangement and containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA) and a control region. Except for the control region (868 bp), one novel larger intergenic (616 bp) was found between nad2 and trnW. Phylogenetic results unambiguously support the monophyly of Phaneropterinae, although the gene order of two Sinochlora species different with other Tettigoniidae species. Using Illumina NGS platforms for mitogenome sequencing will provide rather essential and important DNA molecular data for the further phylogenetic analysis across major ensiferan lineages.