Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)

The complete mitochondrial genome of the European blackfly, Simulium variegatum Meigen, 1818 was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of Chironomus tepperi Skuse, 1889 (Chironomidae) homologous NGS reads were identified and assembled. The genom...

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Detalles Bibliográficos
Autores principales: Day, John C., Gweon, Hyun S., Post, Rory J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Mitocommunication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800089/
https://www.ncbi.nlm.nih.gov/pubmed/33473632
http://dx.doi.org/10.1080/23802359.2016.1209091
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author Day, John C.
Gweon, Hyun S.
Post, Rory J.
author_facet Day, John C.
Gweon, Hyun S.
Post, Rory J.
author_sort Day, John C.
collection PubMed
description The complete mitochondrial genome of the European blackfly, Simulium variegatum Meigen, 1818 was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of Chironomus tepperi Skuse, 1889 (Chironomidae) homologous NGS reads were identified and assembled. The genome is 15,367 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of the ancestral dipteran gene arrangement. The base composition of the genome is A (37.6%), T (35.3%), C (15.8%) and G (11.3%). The control region between 12S rRNA and tRNAIle is composed of 362 bp with no obvious repetitive motifs.
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spelling pubmed-78000892021-01-19 Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae) Day, John C. Gweon, Hyun S. Post, Rory J. Mitochondrial DNA B Resour Mitocommunication The complete mitochondrial genome of the European blackfly, Simulium variegatum Meigen, 1818 was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of Chironomus tepperi Skuse, 1889 (Chironomidae) homologous NGS reads were identified and assembled. The genome is 15,367 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of the ancestral dipteran gene arrangement. The base composition of the genome is A (37.6%), T (35.3%), C (15.8%) and G (11.3%). The control region between 12S rRNA and tRNAIle is composed of 362 bp with no obvious repetitive motifs. Taylor & Francis 2016-11-22 /pmc/articles/PMC7800089/ /pubmed/33473632 http://dx.doi.org/10.1080/23802359.2016.1209091 Text en © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Mitocommunication
Day, John C.
Gweon, Hyun S.
Post, Rory J.
Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)
title Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)
title_full Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)
title_fullStr Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)
title_full_unstemmed Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)
title_short Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)
title_sort sequence and organization of the complete mitochondrial genome of the blackfly simulium variegatum (diptera: simuliidae)
topic Mitocommunication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800089/
https://www.ncbi.nlm.nih.gov/pubmed/33473632
http://dx.doi.org/10.1080/23802359.2016.1209091
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