Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples

We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning. These primers amplified 301–1599 bp DNA fragments in various combinations. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Kumar, Ajit, Ghazi, Mirza Ghazanfar Ullah, Singh, Bhim, Hussain, Syed Ainul, Bhatt, Dinesh, Gupta, Sandeep Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Mito Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800399/
https://www.ncbi.nlm.nih.gov/pubmed/33490438
http://dx.doi.org/10.1080/23802359.2016.1247672
_version_ 1783635344598499328
author Kumar, Ajit
Ghazi, Mirza Ghazanfar Ullah
Singh, Bhim
Hussain, Syed Ainul
Bhatt, Dinesh
Gupta, Sandeep Kumar
author_facet Kumar, Ajit
Ghazi, Mirza Ghazanfar Ullah
Singh, Bhim
Hussain, Syed Ainul
Bhatt, Dinesh
Gupta, Sandeep Kumar
author_sort Kumar, Ajit
collection PubMed
description We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning. These primers amplified 301–1599 bp DNA fragments in various combinations. The method described may assist in the sequencing of either complete gene from moderate to good quality DNA or shorter fragment from degraded DNA.
format Online
Article
Text
id pubmed-7800399
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-78003992021-01-21 Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples Kumar, Ajit Ghazi, Mirza Ghazanfar Ullah Singh, Bhim Hussain, Syed Ainul Bhatt, Dinesh Gupta, Sandeep Kumar Mitochondrial DNA B Resour Mito Communication We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning. These primers amplified 301–1599 bp DNA fragments in various combinations. The method described may assist in the sequencing of either complete gene from moderate to good quality DNA or shorter fragment from degraded DNA. Taylor & Francis 2017-02-06 /pmc/articles/PMC7800399/ /pubmed/33490438 http://dx.doi.org/10.1080/23802359.2016.1247672 Text en © 2017 Wildlife Institute of India. Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Mito Communication
Kumar, Ajit
Ghazi, Mirza Ghazanfar Ullah
Singh, Bhim
Hussain, Syed Ainul
Bhatt, Dinesh
Gupta, Sandeep Kumar
Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples
title Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples
title_full Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples
title_fullStr Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples
title_full_unstemmed Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples
title_short Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples
title_sort conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase i (coi) gene from problematic and decomposed biological samples
topic Mito Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800399/
https://www.ncbi.nlm.nih.gov/pubmed/33490438
http://dx.doi.org/10.1080/23802359.2016.1247672
work_keys_str_mv AT kumarajit conserveprimersforsequencingcompleteungulatemitochondrialcytochromecoxidaseicoigenefromproblematicanddecomposedbiologicalsamples
AT ghazimirzaghazanfarullah conserveprimersforsequencingcompleteungulatemitochondrialcytochromecoxidaseicoigenefromproblematicanddecomposedbiologicalsamples
AT singhbhim conserveprimersforsequencingcompleteungulatemitochondrialcytochromecoxidaseicoigenefromproblematicanddecomposedbiologicalsamples
AT hussainsyedainul conserveprimersforsequencingcompleteungulatemitochondrialcytochromecoxidaseicoigenefromproblematicanddecomposedbiologicalsamples
AT bhattdinesh conserveprimersforsequencingcompleteungulatemitochondrialcytochromecoxidaseicoigenefromproblematicanddecomposedbiologicalsamples
AT guptasandeepkumar conserveprimersforsequencingcompleteungulatemitochondrialcytochromecoxidaseicoigenefromproblematicanddecomposedbiologicalsamples

Ejemplares similares