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Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues

The present study investigated the single nucleotide variants (SNVs) in mitochondrial DNA (mtDNA) of 13 paired gastric cancer tissue samples and seven gastric cancer cell lines using direct sequencing analysis of the MTND5 region. Results showed that nuclear mitochondrial pseudogenes (NUMTs) and mit...

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Autores principales: Li, Lian, Xing, Rui, Cui, Jiantao, Li, Wenmei, Lu, Youyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800514/
https://www.ncbi.nlm.nih.gov/pubmed/33474396
http://dx.doi.org/10.1080/23802359.2018.1501287
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author Li, Lian
Xing, Rui
Cui, Jiantao
Li, Wenmei
Lu, Youyong
author_facet Li, Lian
Xing, Rui
Cui, Jiantao
Li, Wenmei
Lu, Youyong
author_sort Li, Lian
collection PubMed
description The present study investigated the single nucleotide variants (SNVs) in mitochondrial DNA (mtDNA) of 13 paired gastric cancer tissue samples and seven gastric cancer cell lines using direct sequencing analysis of the MTND5 region. Results showed that nuclear mitochondrial pseudogenes (NUMTs) and mitochondrial copy number affected the detection of the SNV frequency in gastric cancer tissue and cell line samples using high-throughput sequencing technique. The heteroplasmic point mutation C12474T and G12835A happened in AGS and BGC823 cell lines, respectively. A total of seven SNVs were found in three paired gastric cancer tissue samples, including five heteroplasmic point mutations (A12406G, C12705T, T12882C, G12501A, and A12584G) and two homoplasmic point mutations (G12561A and C13590T). Gastric cancer tissue sample 16 exhibited the highest SNVs frequency with four SNVs (np 12406, np 12705, np 12882, and np 12501), whereas no SNVs or SNPs were detected in the tissue sample 4. SNP 12705 turned out to be an SNV in gastric cancer tissue sample 16. SNV 12338 detected by exome sequencing approach appeared to be an SNP in this study.
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spelling pubmed-78005142021-01-19 Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues Li, Lian Xing, Rui Cui, Jiantao Li, Wenmei Lu, Youyong Mitochondrial DNA B Resour Mitogenome Announcement The present study investigated the single nucleotide variants (SNVs) in mitochondrial DNA (mtDNA) of 13 paired gastric cancer tissue samples and seven gastric cancer cell lines using direct sequencing analysis of the MTND5 region. Results showed that nuclear mitochondrial pseudogenes (NUMTs) and mitochondrial copy number affected the detection of the SNV frequency in gastric cancer tissue and cell line samples using high-throughput sequencing technique. The heteroplasmic point mutation C12474T and G12835A happened in AGS and BGC823 cell lines, respectively. A total of seven SNVs were found in three paired gastric cancer tissue samples, including five heteroplasmic point mutations (A12406G, C12705T, T12882C, G12501A, and A12584G) and two homoplasmic point mutations (G12561A and C13590T). Gastric cancer tissue sample 16 exhibited the highest SNVs frequency with four SNVs (np 12406, np 12705, np 12882, and np 12501), whereas no SNVs or SNPs were detected in the tissue sample 4. SNP 12705 turned out to be an SNV in gastric cancer tissue sample 16. SNV 12338 detected by exome sequencing approach appeared to be an SNP in this study. Taylor & Francis 2018-08-29 /pmc/articles/PMC7800514/ /pubmed/33474396 http://dx.doi.org/10.1080/23802359.2018.1501287 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Mitogenome Announcement
Li, Lian
Xing, Rui
Cui, Jiantao
Li, Wenmei
Lu, Youyong
Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues
title Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues
title_full Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues
title_fullStr Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues
title_full_unstemmed Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues
title_short Investigation of frequent somatic mutations of MTND5 gene in gastric cancer cell lines and tissues
title_sort investigation of frequent somatic mutations of mtnd5 gene in gastric cancer cell lines and tissues
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800514/
https://www.ncbi.nlm.nih.gov/pubmed/33474396
http://dx.doi.org/10.1080/23802359.2018.1501287
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