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Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing

Mitochondrial DNA (mtDNA) plays a key role in the development of a competent oocyte. Mutations of the mitochondrial genome lead to an altered energetic metabolism with negative effects on oocyte developmental competence. In this study, mtDNA heteroplasmy at an intra-oocyte level and between the diff...

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Autores principales: Ancora, Massimo, Orsini, Massimiliano, Colosimo, Alessia, Russo, Valentina, Marcacci, Maurilia, De Santo, Maria, D’Aurora, Marco, Stuppia, Liborio, Gatta, Valentina, Barboni, Barbara, Cammà, Cesare, Mattioli, Mauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800564/
https://www.ncbi.nlm.nih.gov/pubmed/33473893
http://dx.doi.org/10.1080/23802359.2017.1365634
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author Ancora, Massimo
Orsini, Massimiliano
Colosimo, Alessia
Russo, Valentina
Marcacci, Maurilia
De Santo, Maria
D’Aurora, Marco
Stuppia, Liborio
Gatta, Valentina
Barboni, Barbara
Cammà, Cesare
Mattioli, Mauro
author_facet Ancora, Massimo
Orsini, Massimiliano
Colosimo, Alessia
Russo, Valentina
Marcacci, Maurilia
De Santo, Maria
D’Aurora, Marco
Stuppia, Liborio
Gatta, Valentina
Barboni, Barbara
Cammà, Cesare
Mattioli, Mauro
author_sort Ancora, Massimo
collection PubMed
description Mitochondrial DNA (mtDNA) plays a key role in the development of a competent oocyte. Mutations of the mitochondrial genome lead to an altered energetic metabolism with negative effects on oocyte developmental competence. In this study, mtDNA heteroplasmy at an intra-oocyte level and between the different analyzed human oocytes (n = 12) was identified by a next-generation sequencing (NGS) protocol previously developed by this research group and submitted to GenBank. This method highlighted, in particular, variants in the genes involved in the respiratory chain providing a direct indication of the cell-specific damage within the mitochondrial genome as predictor of the oocyte quality.
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spelling pubmed-78005642021-01-19 Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing Ancora, Massimo Orsini, Massimiliano Colosimo, Alessia Russo, Valentina Marcacci, Maurilia De Santo, Maria D’Aurora, Marco Stuppia, Liborio Gatta, Valentina Barboni, Barbara Cammà, Cesare Mattioli, Mauro Mitochondrial DNA B Resour Mitogenome Announcement Mitochondrial DNA (mtDNA) plays a key role in the development of a competent oocyte. Mutations of the mitochondrial genome lead to an altered energetic metabolism with negative effects on oocyte developmental competence. In this study, mtDNA heteroplasmy at an intra-oocyte level and between the different analyzed human oocytes (n = 12) was identified by a next-generation sequencing (NGS) protocol previously developed by this research group and submitted to GenBank. This method highlighted, in particular, variants in the genes involved in the respiratory chain providing a direct indication of the cell-specific damage within the mitochondrial genome as predictor of the oocyte quality. Taylor & Francis 2017-08-17 /pmc/articles/PMC7800564/ /pubmed/33473893 http://dx.doi.org/10.1080/23802359.2017.1365634 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Mitogenome Announcement
Ancora, Massimo
Orsini, Massimiliano
Colosimo, Alessia
Russo, Valentina
Marcacci, Maurilia
De Santo, Maria
D’Aurora, Marco
Stuppia, Liborio
Gatta, Valentina
Barboni, Barbara
Cammà, Cesare
Mattioli, Mauro
Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing
title Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing
title_full Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing
title_fullStr Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing
title_full_unstemmed Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing
title_short Mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing
title_sort mitochondrial heteroplasmy profiling in single human oocytes by next-generation sequencing
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800564/
https://www.ncbi.nlm.nih.gov/pubmed/33473893
http://dx.doi.org/10.1080/23802359.2017.1365634
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