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A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice
Preclinical studies of human cellular and tissue-based products (HCT/Ps) for transplantation therapy of type 1 diabetes mellitus (T1DM) necessarily involve animal models, particularly mouse models of diabetes induced by streptozotocin (STZ). These models should mimic the clinical and metabolic manif...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
A.I. Gordeyev
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800597/ https://www.ncbi.nlm.nih.gov/pubmed/33456981 http://dx.doi.org/10.32607/actanaturae.11202 |
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author | Gvazava, I. G. Kosykh, A. V. Rogovaya, O. S. Popova, O. P. Sobyanin, K. A. Khrushchev, A. C. Timofeev, A. V. Vorotelyak, E. A. |
author_facet | Gvazava, I. G. Kosykh, A. V. Rogovaya, O. S. Popova, O. P. Sobyanin, K. A. Khrushchev, A. C. Timofeev, A. V. Vorotelyak, E. A. |
author_sort | Gvazava, I. G. |
collection | PubMed |
description | Preclinical studies of human cellular and tissue-based products (HCT/Ps) for transplantation therapy of type 1 diabetes mellitus (T1DM) necessarily involve animal models, particularly mouse models of diabetes induced by streptozotocin (STZ). These models should mimic the clinical and metabolic manifestations of T1DM in humans (face validity) and be similar to T1DM in terms of the pathogenetic mechanism (construct validity). Furthermore, since HCT/Ps contain human cells, modeling of diabetes in immune-deficient animals is obligatory. Here we describe the most simplified diabetes model in Nude mice. Diabetes was induced in 31 males by a single intraperitoneal injection of STZ in normal saline at a medium-to-high dose of 150 mg/kg body weight. Fourteen control animals received only saline. Non-fasting plasma glucose (PG) levels were measured periodically for 50 days. All STZ-treated mice survived beyond 50 days. By day 15 after STZ administration, 22 of 31 (71%) mice developed stable diabetes based on the following criteria: (1) non-fasting PG ≥ 15 mmol/L on consecutive measurements up until day 50; (2) no diabetes remission. The mean non-fasting PG in mice with stable diabetes over the period of 35 days was equal to 25.7 mmol/L. On day 50, mean plasma insulin concentration, mean pancreatic insulin content, and the average number of β-cells in pancreatic islets were 2.6, 8.4, and 50 times lower, respectively, than in the control animals. We consider that our Nude mouse model of diabetes meets face validity and construct validity criteria and can be used in preclinical studies of HCT/Ps. |
format | Online Article Text |
id | pubmed-7800597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | A.I. Gordeyev |
record_format | MEDLINE/PubMed |
spelling | pubmed-78005972021-01-15 A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice Gvazava, I. G. Kosykh, A. V. Rogovaya, O. S. Popova, O. P. Sobyanin, K. A. Khrushchev, A. C. Timofeev, A. V. Vorotelyak, E. A. Acta Naturae Research Article Preclinical studies of human cellular and tissue-based products (HCT/Ps) for transplantation therapy of type 1 diabetes mellitus (T1DM) necessarily involve animal models, particularly mouse models of diabetes induced by streptozotocin (STZ). These models should mimic the clinical and metabolic manifestations of T1DM in humans (face validity) and be similar to T1DM in terms of the pathogenetic mechanism (construct validity). Furthermore, since HCT/Ps contain human cells, modeling of diabetes in immune-deficient animals is obligatory. Here we describe the most simplified diabetes model in Nude mice. Diabetes was induced in 31 males by a single intraperitoneal injection of STZ in normal saline at a medium-to-high dose of 150 mg/kg body weight. Fourteen control animals received only saline. Non-fasting plasma glucose (PG) levels were measured periodically for 50 days. All STZ-treated mice survived beyond 50 days. By day 15 after STZ administration, 22 of 31 (71%) mice developed stable diabetes based on the following criteria: (1) non-fasting PG ≥ 15 mmol/L on consecutive measurements up until day 50; (2) no diabetes remission. The mean non-fasting PG in mice with stable diabetes over the period of 35 days was equal to 25.7 mmol/L. On day 50, mean plasma insulin concentration, mean pancreatic insulin content, and the average number of β-cells in pancreatic islets were 2.6, 8.4, and 50 times lower, respectively, than in the control animals. We consider that our Nude mouse model of diabetes meets face validity and construct validity criteria and can be used in preclinical studies of HCT/Ps. A.I. Gordeyev 2020 /pmc/articles/PMC7800597/ /pubmed/33456981 http://dx.doi.org/10.32607/actanaturae.11202 Text en Copyright ® 2020 National Research University Higher School of Economics. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gvazava, I. G. Kosykh, A. V. Rogovaya, O. S. Popova, O. P. Sobyanin, K. A. Khrushchev, A. C. Timofeev, A. V. Vorotelyak, E. A. A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice |
title | A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice |
title_full | A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice |
title_fullStr | A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice |
title_full_unstemmed | A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice |
title_short | A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice |
title_sort | simplified streptozotocin-induced diabetes model in nude mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800597/ https://www.ncbi.nlm.nih.gov/pubmed/33456981 http://dx.doi.org/10.32607/actanaturae.11202 |
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