A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)

Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of the mitochondrial cytochrome c oxidase subunit I gene (COI) amplification has been quite variable when it comes to Cephalopoda. Some species in this class such as Sthenoteuthi...

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Autores principales: Xu, Lei, Huang, Qi, Xu, Shaolin, Wang, Xuehui, Zhang, Peng, Xu, Liang, Du, Feiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Mito Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800671/
https://www.ncbi.nlm.nih.gov/pubmed/33473855
http://dx.doi.org/10.1080/23802359.2017.1357439
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author Xu, Lei
Huang, Qi
Xu, Shaolin
Wang, Xuehui
Zhang, Peng
Xu, Liang
Du, Feiyan
author_facet Xu, Lei
Huang, Qi
Xu, Shaolin
Wang, Xuehui
Zhang, Peng
Xu, Liang
Du, Feiyan
author_sort Xu, Lei
collection PubMed
description Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of the mitochondrial cytochrome c oxidase subunit I gene (COI) amplification has been quite variable when it comes to Cephalopoda. Some species in this class such as Sthenoteuthis oualaniensis seem to be more difficult to amplify COI than others due to failed amplifications with universal primer and lack of specific set of primers. In this study, we developed new Sthenoteuthis – specific primer set, which significantly increased average amplification success. The new primer set will aid the recovery of barcodes from this difficult group and facilitate further studies in phylogeny and cryptic diversity of Sthenoteuthis oualaniensis.
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spelling pubmed-78006712021-01-19 A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis) Xu, Lei Huang, Qi Xu, Shaolin Wang, Xuehui Zhang, Peng Xu, Liang Du, Feiyan Mitochondrial DNA B Resour Mito Communication Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of the mitochondrial cytochrome c oxidase subunit I gene (COI) amplification has been quite variable when it comes to Cephalopoda. Some species in this class such as Sthenoteuthis oualaniensis seem to be more difficult to amplify COI than others due to failed amplifications with universal primer and lack of specific set of primers. In this study, we developed new Sthenoteuthis – specific primer set, which significantly increased average amplification success. The new primer set will aid the recovery of barcodes from this difficult group and facilitate further studies in phylogeny and cryptic diversity of Sthenoteuthis oualaniensis. Taylor & Francis 2017-07-25 /pmc/articles/PMC7800671/ /pubmed/33473855 http://dx.doi.org/10.1080/23802359.2017.1357439 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Mito Communication
Xu, Lei
Huang, Qi
Xu, Shaolin
Wang, Xuehui
Zhang, Peng
Xu, Liang
Du, Feiyan
A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)
title A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)
title_full A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)
title_fullStr A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)
title_full_unstemmed A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)
title_short A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)
title_sort new set of primers for coi amplification from purpleback flying squid (sthenoteuthis oualaniensis)
topic Mito Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800671/
https://www.ncbi.nlm.nih.gov/pubmed/33473855
http://dx.doi.org/10.1080/23802359.2017.1357439
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