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The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing
The Thomas’s horseshoe bat (Rhinolophus thomasi) is restricted to southeast Asia and few studies have been conducted on this species. Here we characterized the complete mitochondrial genome of R. thomasi using next-generation sequencing and Sanger sequencing. The whole mitogenome is 16,899 bp in len...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800769/ https://www.ncbi.nlm.nih.gov/pubmed/33473693 http://dx.doi.org/10.1080/23802359.2016.1266707 |
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author | Xing, Yutong Mao, Xiuguang |
author_facet | Xing, Yutong Mao, Xiuguang |
author_sort | Xing, Yutong |
collection | PubMed |
description | The Thomas’s horseshoe bat (Rhinolophus thomasi) is restricted to southeast Asia and few studies have been conducted on this species. Here we characterized the complete mitochondrial genome of R. thomasi using next-generation sequencing and Sanger sequencing. The whole mitogenome is 16,899 bp in length and contains 22 tRNA, 2 rRNA, 13 protein-coding genes and a non-coding control region. The tissue used in this study was taken from a sample collected 13 years ago and the genomic DNA was seriously degraded. Our study adds to a growing number of studies indicating that next-generation sequencing is powerful in generating genetic/genomic resources from museum samples. |
format | Online Article Text |
id | pubmed-7800769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78007692021-01-19 The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing Xing, Yutong Mao, Xiuguang Mitochondrial DNA B Resour Mitogenome Announcement The Thomas’s horseshoe bat (Rhinolophus thomasi) is restricted to southeast Asia and few studies have been conducted on this species. Here we characterized the complete mitochondrial genome of R. thomasi using next-generation sequencing and Sanger sequencing. The whole mitogenome is 16,899 bp in length and contains 22 tRNA, 2 rRNA, 13 protein-coding genes and a non-coding control region. The tissue used in this study was taken from a sample collected 13 years ago and the genomic DNA was seriously degraded. Our study adds to a growing number of studies indicating that next-generation sequencing is powerful in generating genetic/genomic resources from museum samples. Taylor & Francis 2016-12-23 /pmc/articles/PMC7800769/ /pubmed/33473693 http://dx.doi.org/10.1080/23802359.2016.1266707 Text en © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Mitogenome Announcement Xing, Yutong Mao, Xiuguang The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing |
title | The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing |
title_full | The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing |
title_fullStr | The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing |
title_full_unstemmed | The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing |
title_short | The complete mitochondrial genome of the Thomas’s horseshoe bat (Rhinolophus thomasi) using next-generation sequencing and Sanger sequencing |
title_sort | complete mitochondrial genome of the thomas’s horseshoe bat (rhinolophus thomasi) using next-generation sequencing and sanger sequencing |
topic | Mitogenome Announcement |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800769/ https://www.ncbi.nlm.nih.gov/pubmed/33473693 http://dx.doi.org/10.1080/23802359.2016.1266707 |
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