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Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening
A series of stable and ready-to-use bioinks have been developed based on the xeno-free and tunable hydrogel (VitroGel) system. Cell laden scaffold fabrication with optimized polysaccharide-based inks demonstrated that Ink H4 and RGD modified Ink H4-RGD had excellent rheological properties. Both bioi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801509/ https://www.ncbi.nlm.nih.gov/pubmed/33431915 http://dx.doi.org/10.1038/s41598-020-79325-8 |
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author | Gebeyehu, Aragaw Surapaneni, Sunil Kumar Huang, John Mondal, Arindam Wang, Vivian Ziwen Haruna, Nana Fatima Bagde, Arvind Arthur, Peggy Kutlehria, Shallu Patel, Nil Rishi, Arun K. Singh, Mandip |
author_facet | Gebeyehu, Aragaw Surapaneni, Sunil Kumar Huang, John Mondal, Arindam Wang, Vivian Ziwen Haruna, Nana Fatima Bagde, Arvind Arthur, Peggy Kutlehria, Shallu Patel, Nil Rishi, Arun K. Singh, Mandip |
author_sort | Gebeyehu, Aragaw |
collection | PubMed |
description | A series of stable and ready-to-use bioinks have been developed based on the xeno-free and tunable hydrogel (VitroGel) system. Cell laden scaffold fabrication with optimized polysaccharide-based inks demonstrated that Ink H4 and RGD modified Ink H4-RGD had excellent rheological properties. Both bioinks were printable with 25–40 kPa extrusion pressure, showed 90% cell viability, shear-thinning and rapid shear recovery properties making them feasible for extrusion bioprinting without UV curing or temperature adjustment. Ink H4-RGD showed printability between 20 and 37 °C and the scaffolds remained stable for 15 days at temperature of 37 °C. 3D printed non-small-cell lung cancer (NSCLC) patient derived xenograft cells (PDCs) showed rapid spheroid growth of size around 500 µm in diameter and tumor microenvironment formation within 7 days. IC(50) values demonstrated higher resistance of 3D spheroids to docetaxel (DTX), doxorubicin (DOX) and erlotinib compared to 2D monolayers of NSCLC-PDX, wild type triple negative breast cancer (MDA-MB-231 WT) and lung adenocarcinoma (HCC-827) cells. Results of flow property, shape fidelity, scaffold stability and biocompatibility of H4-RGD suggest that this hydrogel could be considered for 3D cell bioprinting and also for in-vitro tumor microenvironment development for high throughput screening of various anti-cancer drugs. |
format | Online Article Text |
id | pubmed-7801509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78015092021-01-12 Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening Gebeyehu, Aragaw Surapaneni, Sunil Kumar Huang, John Mondal, Arindam Wang, Vivian Ziwen Haruna, Nana Fatima Bagde, Arvind Arthur, Peggy Kutlehria, Shallu Patel, Nil Rishi, Arun K. Singh, Mandip Sci Rep Article A series of stable and ready-to-use bioinks have been developed based on the xeno-free and tunable hydrogel (VitroGel) system. Cell laden scaffold fabrication with optimized polysaccharide-based inks demonstrated that Ink H4 and RGD modified Ink H4-RGD had excellent rheological properties. Both bioinks were printable with 25–40 kPa extrusion pressure, showed 90% cell viability, shear-thinning and rapid shear recovery properties making them feasible for extrusion bioprinting without UV curing or temperature adjustment. Ink H4-RGD showed printability between 20 and 37 °C and the scaffolds remained stable for 15 days at temperature of 37 °C. 3D printed non-small-cell lung cancer (NSCLC) patient derived xenograft cells (PDCs) showed rapid spheroid growth of size around 500 µm in diameter and tumor microenvironment formation within 7 days. IC(50) values demonstrated higher resistance of 3D spheroids to docetaxel (DTX), doxorubicin (DOX) and erlotinib compared to 2D monolayers of NSCLC-PDX, wild type triple negative breast cancer (MDA-MB-231 WT) and lung adenocarcinoma (HCC-827) cells. Results of flow property, shape fidelity, scaffold stability and biocompatibility of H4-RGD suggest that this hydrogel could be considered for 3D cell bioprinting and also for in-vitro tumor microenvironment development for high throughput screening of various anti-cancer drugs. Nature Publishing Group UK 2021-01-11 /pmc/articles/PMC7801509/ /pubmed/33431915 http://dx.doi.org/10.1038/s41598-020-79325-8 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gebeyehu, Aragaw Surapaneni, Sunil Kumar Huang, John Mondal, Arindam Wang, Vivian Ziwen Haruna, Nana Fatima Bagde, Arvind Arthur, Peggy Kutlehria, Shallu Patel, Nil Rishi, Arun K. Singh, Mandip Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening |
title | Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening |
title_full | Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening |
title_fullStr | Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening |
title_full_unstemmed | Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening |
title_short | Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening |
title_sort | polysaccharide hydrogel based 3d printed tumor models for chemotherapeutic drug screening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801509/ https://www.ncbi.nlm.nih.gov/pubmed/33431915 http://dx.doi.org/10.1038/s41598-020-79325-8 |
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