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Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction

Throughout infection, plant-parasitic nematodes activate a complex host defense response that will regulate their development and aggressiveness. Oxylipins—lipophilic signaling molecules—are part of this complex, performing a fundamental role in regulating plant development and immunity. At the same...

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Autores principales: Fitoussi, Nathalia, Borrego, Eli, Kolomiets, Michael V., Qing, Xue, Bucki, Patricia, Sela, Noa, Belausov, Eduard, Braun Miyara, Sigal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801703/
https://www.ncbi.nlm.nih.gov/pubmed/33431951
http://dx.doi.org/10.1038/s41598-020-79432-6
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author Fitoussi, Nathalia
Borrego, Eli
Kolomiets, Michael V.
Qing, Xue
Bucki, Patricia
Sela, Noa
Belausov, Eduard
Braun Miyara, Sigal
author_facet Fitoussi, Nathalia
Borrego, Eli
Kolomiets, Michael V.
Qing, Xue
Bucki, Patricia
Sela, Noa
Belausov, Eduard
Braun Miyara, Sigal
author_sort Fitoussi, Nathalia
collection PubMed
description Throughout infection, plant-parasitic nematodes activate a complex host defense response that will regulate their development and aggressiveness. Oxylipins—lipophilic signaling molecules—are part of this complex, performing a fundamental role in regulating plant development and immunity. At the same time, the sedentary root-knot nematode Meloidogyne spp. secretes numerous effectors that play key roles during invasion and migration, supporting construction and maintenance of nematodes' feeding sites. Herein, comprehensive oxylipin profiling of tomato roots, performed using LC–MS/MS, indicated strong and early responses of many oxylipins following root-knot nematode infection. To identify genes that might respond to the lipidomic defense pathway mediated through oxylipins, RNA-Seq was performed by exposing Meloidogyne javanica second-stage juveniles to tomato protoplasts and the oxylipin 9-HOT, one of the early-induced oxylipins in tomato roots upon nematode infection. A total of 7512 differentially expressed genes were identified. To target putative effectors, we sought differentially expressed genes carrying a predicted secretion signal peptide. Among these, several were homologous with known effectors in other nematode species; other unknown, potentially secreted proteins may have a role as root-knot nematode effectors that are induced by plant lipid signals. These include effectors associated with distortion of the plant immune response or manipulating signal transduction mediated by lipid signals. Other effectors are implicated in cell wall degradation or ROS detoxification at the plant–nematode interface. Being an integral part of the plant's defense response, oxylipins might be placed as important signaling molecules underlying nematode parasitism.
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spelling pubmed-78017032021-01-13 Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction Fitoussi, Nathalia Borrego, Eli Kolomiets, Michael V. Qing, Xue Bucki, Patricia Sela, Noa Belausov, Eduard Braun Miyara, Sigal Sci Rep Article Throughout infection, plant-parasitic nematodes activate a complex host defense response that will regulate their development and aggressiveness. Oxylipins—lipophilic signaling molecules—are part of this complex, performing a fundamental role in regulating plant development and immunity. At the same time, the sedentary root-knot nematode Meloidogyne spp. secretes numerous effectors that play key roles during invasion and migration, supporting construction and maintenance of nematodes' feeding sites. Herein, comprehensive oxylipin profiling of tomato roots, performed using LC–MS/MS, indicated strong and early responses of many oxylipins following root-knot nematode infection. To identify genes that might respond to the lipidomic defense pathway mediated through oxylipins, RNA-Seq was performed by exposing Meloidogyne javanica second-stage juveniles to tomato protoplasts and the oxylipin 9-HOT, one of the early-induced oxylipins in tomato roots upon nematode infection. A total of 7512 differentially expressed genes were identified. To target putative effectors, we sought differentially expressed genes carrying a predicted secretion signal peptide. Among these, several were homologous with known effectors in other nematode species; other unknown, potentially secreted proteins may have a role as root-knot nematode effectors that are induced by plant lipid signals. These include effectors associated with distortion of the plant immune response or manipulating signal transduction mediated by lipid signals. Other effectors are implicated in cell wall degradation or ROS detoxification at the plant–nematode interface. Being an integral part of the plant's defense response, oxylipins might be placed as important signaling molecules underlying nematode parasitism. Nature Publishing Group UK 2021-01-11 /pmc/articles/PMC7801703/ /pubmed/33431951 http://dx.doi.org/10.1038/s41598-020-79432-6 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fitoussi, Nathalia
Borrego, Eli
Kolomiets, Michael V.
Qing, Xue
Bucki, Patricia
Sela, Noa
Belausov, Eduard
Braun Miyara, Sigal
Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction
title Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction
title_full Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction
title_fullStr Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction
title_full_unstemmed Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction
title_short Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction
title_sort oxylipins are implicated as communication signals in tomato–root-knot nematode (meloidogyne javanica) interaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801703/
https://www.ncbi.nlm.nih.gov/pubmed/33431951
http://dx.doi.org/10.1038/s41598-020-79432-6
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