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Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration

Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor di...

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Autores principales: Sudharsan, Raghavi, Murgiano, Leonardo, Tang, Hsin-Yao, Olsen, Timothy W., Chavali, Venkata R. M., Aguirre, Gustavo D., Beltran, William A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801730/
https://www.ncbi.nlm.nih.gov/pubmed/33432105
http://dx.doi.org/10.1038/s41598-020-80691-6
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author Sudharsan, Raghavi
Murgiano, Leonardo
Tang, Hsin-Yao
Olsen, Timothy W.
Chavali, Venkata R. M.
Aguirre, Gustavo D.
Beltran, William A.
author_facet Sudharsan, Raghavi
Murgiano, Leonardo
Tang, Hsin-Yao
Olsen, Timothy W.
Chavali, Venkata R. M.
Aguirre, Gustavo D.
Beltran, William A.
author_sort Sudharsan, Raghavi
collection PubMed
description Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor disease. In the present study, we have identified the expression of a short PRL isoform that lacks exon 1 in canine retinas and analyzed the time-course of expression and localization of this isoform in the retinas of these two models. Using laser capture microdissection to isolate RNA from each of the retinal cellular layers, we found by qPCR that this short PRL isoform is expressed in photoreceptors of degenerating retinas. We confirmed by in situ hybridization that its expression is localized to the outer nuclear layer and begins shortly after the onset of disease at the time of peak photoreceptor cell death in both models. PRL protein was also detected only in mutant dog retinas. Our results call for further investigations into the role of this novel PRL isoform in retinal degeneration.
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spelling pubmed-78017302021-01-13 Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration Sudharsan, Raghavi Murgiano, Leonardo Tang, Hsin-Yao Olsen, Timothy W. Chavali, Venkata R. M. Aguirre, Gustavo D. Beltran, William A. Sci Rep Article Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor disease. In the present study, we have identified the expression of a short PRL isoform that lacks exon 1 in canine retinas and analyzed the time-course of expression and localization of this isoform in the retinas of these two models. Using laser capture microdissection to isolate RNA from each of the retinal cellular layers, we found by qPCR that this short PRL isoform is expressed in photoreceptors of degenerating retinas. We confirmed by in situ hybridization that its expression is localized to the outer nuclear layer and begins shortly after the onset of disease at the time of peak photoreceptor cell death in both models. PRL protein was also detected only in mutant dog retinas. Our results call for further investigations into the role of this novel PRL isoform in retinal degeneration. Nature Publishing Group UK 2021-01-11 /pmc/articles/PMC7801730/ /pubmed/33432105 http://dx.doi.org/10.1038/s41598-020-80691-6 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sudharsan, Raghavi
Murgiano, Leonardo
Tang, Hsin-Yao
Olsen, Timothy W.
Chavali, Venkata R. M.
Aguirre, Gustavo D.
Beltran, William A.
Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_full Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_fullStr Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_full_unstemmed Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_short Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_sort short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801730/
https://www.ncbi.nlm.nih.gov/pubmed/33432105
http://dx.doi.org/10.1038/s41598-020-80691-6
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