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Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor di...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801730/ https://www.ncbi.nlm.nih.gov/pubmed/33432105 http://dx.doi.org/10.1038/s41598-020-80691-6 |
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author | Sudharsan, Raghavi Murgiano, Leonardo Tang, Hsin-Yao Olsen, Timothy W. Chavali, Venkata R. M. Aguirre, Gustavo D. Beltran, William A. |
author_facet | Sudharsan, Raghavi Murgiano, Leonardo Tang, Hsin-Yao Olsen, Timothy W. Chavali, Venkata R. M. Aguirre, Gustavo D. Beltran, William A. |
author_sort | Sudharsan, Raghavi |
collection | PubMed |
description | Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor disease. In the present study, we have identified the expression of a short PRL isoform that lacks exon 1 in canine retinas and analyzed the time-course of expression and localization of this isoform in the retinas of these two models. Using laser capture microdissection to isolate RNA from each of the retinal cellular layers, we found by qPCR that this short PRL isoform is expressed in photoreceptors of degenerating retinas. We confirmed by in situ hybridization that its expression is localized to the outer nuclear layer and begins shortly after the onset of disease at the time of peak photoreceptor cell death in both models. PRL protein was also detected only in mutant dog retinas. Our results call for further investigations into the role of this novel PRL isoform in retinal degeneration. |
format | Online Article Text |
id | pubmed-7801730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78017302021-01-13 Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration Sudharsan, Raghavi Murgiano, Leonardo Tang, Hsin-Yao Olsen, Timothy W. Chavali, Venkata R. M. Aguirre, Gustavo D. Beltran, William A. Sci Rep Article Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor disease. In the present study, we have identified the expression of a short PRL isoform that lacks exon 1 in canine retinas and analyzed the time-course of expression and localization of this isoform in the retinas of these two models. Using laser capture microdissection to isolate RNA from each of the retinal cellular layers, we found by qPCR that this short PRL isoform is expressed in photoreceptors of degenerating retinas. We confirmed by in situ hybridization that its expression is localized to the outer nuclear layer and begins shortly after the onset of disease at the time of peak photoreceptor cell death in both models. PRL protein was also detected only in mutant dog retinas. Our results call for further investigations into the role of this novel PRL isoform in retinal degeneration. Nature Publishing Group UK 2021-01-11 /pmc/articles/PMC7801730/ /pubmed/33432105 http://dx.doi.org/10.1038/s41598-020-80691-6 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Sudharsan, Raghavi Murgiano, Leonardo Tang, Hsin-Yao Olsen, Timothy W. Chavali, Venkata R. M. Aguirre, Gustavo D. Beltran, William A. Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration |
title | Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration |
title_full | Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration |
title_fullStr | Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration |
title_full_unstemmed | Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration |
title_short | Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration |
title_sort | short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801730/ https://www.ncbi.nlm.nih.gov/pubmed/33432105 http://dx.doi.org/10.1038/s41598-020-80691-6 |
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