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TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137
BACKGROUND: Acute myeloid leukemia (AML) is a ubiquitous malignancy that occurs in the hematological system. Tripartite motif-containing 25 (TRIM25) has been found to be involved in various carcinomas comprising AML. However, the function and underlying causative role of TRIM25 in AML are still obsc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801882/ https://www.ncbi.nlm.nih.gov/pubmed/33506106 http://dx.doi.org/10.1515/med-2021-0003 |
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author | Wang, Sheng Zhang, Bang Shuo Yang, Yi Li, Ying Lv, Jing Long Cheng, Yu |
author_facet | Wang, Sheng Zhang, Bang Shuo Yang, Yi Li, Ying Lv, Jing Long Cheng, Yu |
author_sort | Wang, Sheng |
collection | PubMed |
description | BACKGROUND: Acute myeloid leukemia (AML) is a ubiquitous malignancy that occurs in the hematological system. Tripartite motif-containing 25 (TRIM25) has been found to be involved in various carcinomas comprising AML. However, the function and underlying causative role of TRIM25 in AML are still obscure. METHODS AND MATERIALS: Quantitative real-time polymerase chain reaction (qPCR) was used for assaying TRIM25 and miR-137 expression in AML samples and cells. CCK-8 assay, Calcein-acetoxymethylester/propidium iodide staining, and Transwell assay were adopted to assay cell proliferation, invasion, and migration. Dual-luciferase reporter experiment was used for analyzing the interaction of TRIM25 with miR-137. Western blot was used for assaying protein expression levels. RESULTS: This study confirmed that TRIM25 expression was upregulated in AML samples and cell lines, whereas miR-137 expression was downregulated. Overexpression of TRIM25 significantly contributed to AML cell’s proliferation, invasion, and migration, whereas knockdown exerted the opposite effect. In addition, TRIM25 was a downstream target of miR-137 in AML cells and negatively modulated by miR-137. CONCLUSION: TRIM25 was targeted and regulated by miR-137, exerted a carcinogenic function in AML, and could be used as a latent biomarker and a treatment target for AML. |
format | Online Article Text |
id | pubmed-7801882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-78018822021-01-26 TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137 Wang, Sheng Zhang, Bang Shuo Yang, Yi Li, Ying Lv, Jing Long Cheng, Yu Open Med (Wars) Research Article BACKGROUND: Acute myeloid leukemia (AML) is a ubiquitous malignancy that occurs in the hematological system. Tripartite motif-containing 25 (TRIM25) has been found to be involved in various carcinomas comprising AML. However, the function and underlying causative role of TRIM25 in AML are still obscure. METHODS AND MATERIALS: Quantitative real-time polymerase chain reaction (qPCR) was used for assaying TRIM25 and miR-137 expression in AML samples and cells. CCK-8 assay, Calcein-acetoxymethylester/propidium iodide staining, and Transwell assay were adopted to assay cell proliferation, invasion, and migration. Dual-luciferase reporter experiment was used for analyzing the interaction of TRIM25 with miR-137. Western blot was used for assaying protein expression levels. RESULTS: This study confirmed that TRIM25 expression was upregulated in AML samples and cell lines, whereas miR-137 expression was downregulated. Overexpression of TRIM25 significantly contributed to AML cell’s proliferation, invasion, and migration, whereas knockdown exerted the opposite effect. In addition, TRIM25 was a downstream target of miR-137 in AML cells and negatively modulated by miR-137. CONCLUSION: TRIM25 was targeted and regulated by miR-137, exerted a carcinogenic function in AML, and could be used as a latent biomarker and a treatment target for AML. De Gruyter 2020-12-24 /pmc/articles/PMC7801882/ /pubmed/33506106 http://dx.doi.org/10.1515/med-2021-0003 Text en © 2021 Sheng Wang et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Wang, Sheng Zhang, Bang Shuo Yang, Yi Li, Ying Lv, Jing Long Cheng, Yu TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137 |
title | TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137 |
title_full | TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137 |
title_fullStr | TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137 |
title_full_unstemmed | TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137 |
title_short | TRIM25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microRNA-137 |
title_sort | trim25 contributes to the malignancy of acute myeloid leukemia and is negatively regulated by microrna-137 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801882/ https://www.ncbi.nlm.nih.gov/pubmed/33506106 http://dx.doi.org/10.1515/med-2021-0003 |
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