Cargando…

Antitumor effect and molecular mechanism of fucoidan in NSCLC

BACKGROUND: Fucoidan, a water-soluble polysaccharide, exerts anticoagulant and antiviral functions. It was recently reported that fucoidan also exerts an antitumor function. Lung cancer is one of the most common cancers in the world. The aim of this study was to investigate anti-tumor,apoptosis and...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Xiaohan, Sun, Li, Wei, Xiaojuan, Lu, Haijun, Tan, Ye, Sun, Zhanyi, Jiang, Jinju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802245/
https://www.ncbi.nlm.nih.gov/pubmed/33430854
http://dx.doi.org/10.1186/s12906-020-03191-0
_version_ 1783635732721565696
author Chen, Xiaohan
Sun, Li
Wei, Xiaojuan
Lu, Haijun
Tan, Ye
Sun, Zhanyi
Jiang, Jinju
author_facet Chen, Xiaohan
Sun, Li
Wei, Xiaojuan
Lu, Haijun
Tan, Ye
Sun, Zhanyi
Jiang, Jinju
author_sort Chen, Xiaohan
collection PubMed
description BACKGROUND: Fucoidan, a water-soluble polysaccharide, exerts anticoagulant and antiviral functions. It was recently reported that fucoidan also exerts an antitumor function. Lung cancer is one of the most common cancers in the world. The aim of this study was to investigate anti-tumor,apoptosis and anti-metastasis effects of fucoidan in both cell-based assays and mouse xenograft model, as well as to clarify possible role of m-TOR pathway in the protection. METHODS: In vitro: Different concentrations of fucoidan were given to act on non-small cell lung cancer (NSCLC) cell lines A549 and H1650. The effects of fucoidan on cell proliferation were observed by detecting cyclin expression levels, CCK8 and EDU experiments and cloning experiments. The apoptotic level was detected by flow cytometry and the apoptotic protein level was detected by Westernblot. By detecting the expression of adhesion molecules, the expression of matrix metalloproteinase (MMP) family, and Transwell cell invasion and migration experiment, the effect of fucoidan on cell adhesion, invasion and migration was observed. Meanwhile the effect of fucoidan on angiogenesis was observed by detecting the expression of vascular endothelial growth factor (VEGF). In vivo experiment: An animal model of NSCLC cell mouse subcutaneous xenograft tumor was established to analyze the correlation between the consumption of fucoidan and the size and volume of xenograft tumor through gross observation. Through immunohistochemical staining and immunofluorescence double staining, ki67 and cell adhesion molecules (E-cadherin, N-cadherin and CD31) and VEGF-A in the tumor were detected, and the correlation between the amount of fucoidan and the above indexes was analyzed. RESULTS: Fucoidan inhibited the proliferation and angiogenesis of NSCLC cells via the mTOR pathway and promoted their apoptosis by increasing the Bax/Bcl-2 ratio. Not only that, fucoidan inhibited NSCLC cell invasion via epithelial–mesenchymal transformation (EMT). The mice fed fucoidan exhibited significant reductions in tumor volumes and weights. These indicators (Ki67, VEGF-A,N-cadherin) were decreased and E-cadherin expression was up-regulated in A549 mice that treated with fucoidan. The results showed that fucoidan inhibited tumor proliferation in vivo by affecting the expression of related proteins. CONCLUSION: Fucoidan conveys antitumor effects and our results represent an ideal therapeutic agent for NSCLC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-020-03191-0.
format Online
Article
Text
id pubmed-7802245
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-78022452021-01-13 Antitumor effect and molecular mechanism of fucoidan in NSCLC Chen, Xiaohan Sun, Li Wei, Xiaojuan Lu, Haijun Tan, Ye Sun, Zhanyi Jiang, Jinju BMC Complement Med Ther Research Article BACKGROUND: Fucoidan, a water-soluble polysaccharide, exerts anticoagulant and antiviral functions. It was recently reported that fucoidan also exerts an antitumor function. Lung cancer is one of the most common cancers in the world. The aim of this study was to investigate anti-tumor,apoptosis and anti-metastasis effects of fucoidan in both cell-based assays and mouse xenograft model, as well as to clarify possible role of m-TOR pathway in the protection. METHODS: In vitro: Different concentrations of fucoidan were given to act on non-small cell lung cancer (NSCLC) cell lines A549 and H1650. The effects of fucoidan on cell proliferation were observed by detecting cyclin expression levels, CCK8 and EDU experiments and cloning experiments. The apoptotic level was detected by flow cytometry and the apoptotic protein level was detected by Westernblot. By detecting the expression of adhesion molecules, the expression of matrix metalloproteinase (MMP) family, and Transwell cell invasion and migration experiment, the effect of fucoidan on cell adhesion, invasion and migration was observed. Meanwhile the effect of fucoidan on angiogenesis was observed by detecting the expression of vascular endothelial growth factor (VEGF). In vivo experiment: An animal model of NSCLC cell mouse subcutaneous xenograft tumor was established to analyze the correlation between the consumption of fucoidan and the size and volume of xenograft tumor through gross observation. Through immunohistochemical staining and immunofluorescence double staining, ki67 and cell adhesion molecules (E-cadherin, N-cadherin and CD31) and VEGF-A in the tumor were detected, and the correlation between the amount of fucoidan and the above indexes was analyzed. RESULTS: Fucoidan inhibited the proliferation and angiogenesis of NSCLC cells via the mTOR pathway and promoted their apoptosis by increasing the Bax/Bcl-2 ratio. Not only that, fucoidan inhibited NSCLC cell invasion via epithelial–mesenchymal transformation (EMT). The mice fed fucoidan exhibited significant reductions in tumor volumes and weights. These indicators (Ki67, VEGF-A,N-cadherin) were decreased and E-cadherin expression was up-regulated in A549 mice that treated with fucoidan. The results showed that fucoidan inhibited tumor proliferation in vivo by affecting the expression of related proteins. CONCLUSION: Fucoidan conveys antitumor effects and our results represent an ideal therapeutic agent for NSCLC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-020-03191-0. BioMed Central 2021-01-11 /pmc/articles/PMC7802245/ /pubmed/33430854 http://dx.doi.org/10.1186/s12906-020-03191-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Chen, Xiaohan
Sun, Li
Wei, Xiaojuan
Lu, Haijun
Tan, Ye
Sun, Zhanyi
Jiang, Jinju
Antitumor effect and molecular mechanism of fucoidan in NSCLC
title Antitumor effect and molecular mechanism of fucoidan in NSCLC
title_full Antitumor effect and molecular mechanism of fucoidan in NSCLC
title_fullStr Antitumor effect and molecular mechanism of fucoidan in NSCLC
title_full_unstemmed Antitumor effect and molecular mechanism of fucoidan in NSCLC
title_short Antitumor effect and molecular mechanism of fucoidan in NSCLC
title_sort antitumor effect and molecular mechanism of fucoidan in nsclc
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802245/
https://www.ncbi.nlm.nih.gov/pubmed/33430854
http://dx.doi.org/10.1186/s12906-020-03191-0
work_keys_str_mv AT chenxiaohan antitumoreffectandmolecularmechanismoffucoidaninnsclc
AT sunli antitumoreffectandmolecularmechanismoffucoidaninnsclc
AT weixiaojuan antitumoreffectandmolecularmechanismoffucoidaninnsclc
AT luhaijun antitumoreffectandmolecularmechanismoffucoidaninnsclc
AT tanye antitumoreffectandmolecularmechanismoffucoidaninnsclc
AT sunzhanyi antitumoreffectandmolecularmechanismoffucoidaninnsclc
AT jiangjinju antitumoreffectandmolecularmechanismoffucoidaninnsclc