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Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)

Two winter rapeseed cultivars, “NS” (cold tolerant) and “NF” (cold sensitive), were used to reveal the morphological, physiological, and proteomic characteristics in leaves of plants after treatment at -4°C for 12 h(T1) and 24 h(T2), and at room temperature(T0), to understand the molecular mechanism...

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Detalles Bibliográficos
Autores principales: Mi, Wenbo, Liu, Zigang, Jin, Jiaojiao, Dong, Xiaoyun, Xu, Chunmei, Zou, Ya, Xu, Mingxia, Zheng, Guoqiang, Cao, Xiaodong, Fang, Xinling, Zhao, Caixia, Mi, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802968/
https://www.ncbi.nlm.nih.gov/pubmed/33434207
http://dx.doi.org/10.1371/journal.pone.0243292
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author Mi, Wenbo
Liu, Zigang
Jin, Jiaojiao
Dong, Xiaoyun
Xu, Chunmei
Zou, Ya
Xu, Mingxia
Zheng, Guoqiang
Cao, Xiaodong
Fang, Xinling
Zhao, Caixia
Mi, Chao
author_facet Mi, Wenbo
Liu, Zigang
Jin, Jiaojiao
Dong, Xiaoyun
Xu, Chunmei
Zou, Ya
Xu, Mingxia
Zheng, Guoqiang
Cao, Xiaodong
Fang, Xinling
Zhao, Caixia
Mi, Chao
author_sort Mi, Wenbo
collection PubMed
description Two winter rapeseed cultivars, “NS” (cold tolerant) and “NF” (cold sensitive), were used to reveal the morphological, physiological, and proteomic characteristics in leaves of plants after treatment at -4°C for 12 h(T1) and 24 h(T2), and at room temperature(T0), to understand the molecular mechanisms of cold tolerance. Antioxidant activity and osmotic adjustment ability were higher, and plasma membrane injury was less obvious, in NS than in NF under cold stress. We detected different abundant proteins (DAPs) related to cold tolerance in winter rapeseed through data-independent acquisition (DIA). Compared with NF, A total of 1,235 and 1,543 DAPs were identified in the NSs under T1 and T2, respectively. Compared with NF, 911 proteins were more abundant in NS only after cold treatment. Some of these proteins were related to ROS scavenging through four metabolic pathways: lysine degradation; phenylalanine, tyrosine, and tryptophan; flavonoid biosynthesis; and ubiquinone and other terpenoid-quinone biosynthesis. Analysis of these proteins in the four candidate pathways revealed that they were rapidly accumulated to quickly enhance ROS scavenging and improve the cold tolerance of NS. These proteins were noticeably more abundant during the early stage of cold stress, which was critical for avoiding ROS damage.
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spelling pubmed-78029682021-01-25 Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.) Mi, Wenbo Liu, Zigang Jin, Jiaojiao Dong, Xiaoyun Xu, Chunmei Zou, Ya Xu, Mingxia Zheng, Guoqiang Cao, Xiaodong Fang, Xinling Zhao, Caixia Mi, Chao PLoS One Research Article Two winter rapeseed cultivars, “NS” (cold tolerant) and “NF” (cold sensitive), were used to reveal the morphological, physiological, and proteomic characteristics in leaves of plants after treatment at -4°C for 12 h(T1) and 24 h(T2), and at room temperature(T0), to understand the molecular mechanisms of cold tolerance. Antioxidant activity and osmotic adjustment ability were higher, and plasma membrane injury was less obvious, in NS than in NF under cold stress. We detected different abundant proteins (DAPs) related to cold tolerance in winter rapeseed through data-independent acquisition (DIA). Compared with NF, A total of 1,235 and 1,543 DAPs were identified in the NSs under T1 and T2, respectively. Compared with NF, 911 proteins were more abundant in NS only after cold treatment. Some of these proteins were related to ROS scavenging through four metabolic pathways: lysine degradation; phenylalanine, tyrosine, and tryptophan; flavonoid biosynthesis; and ubiquinone and other terpenoid-quinone biosynthesis. Analysis of these proteins in the four candidate pathways revealed that they were rapidly accumulated to quickly enhance ROS scavenging and improve the cold tolerance of NS. These proteins were noticeably more abundant during the early stage of cold stress, which was critical for avoiding ROS damage. Public Library of Science 2021-01-12 /pmc/articles/PMC7802968/ /pubmed/33434207 http://dx.doi.org/10.1371/journal.pone.0243292 Text en © 2021 Mi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mi, Wenbo
Liu, Zigang
Jin, Jiaojiao
Dong, Xiaoyun
Xu, Chunmei
Zou, Ya
Xu, Mingxia
Zheng, Guoqiang
Cao, Xiaodong
Fang, Xinling
Zhao, Caixia
Mi, Chao
Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)
title Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)
title_full Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)
title_fullStr Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)
title_full_unstemmed Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)
title_short Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)
title_sort comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ros scavenging in winter rapeseed (brassica napus l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802968/
https://www.ncbi.nlm.nih.gov/pubmed/33434207
http://dx.doi.org/10.1371/journal.pone.0243292
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