Cargando…
Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats
Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, th...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803155/ https://www.ncbi.nlm.nih.gov/pubmed/33488747 http://dx.doi.org/10.1155/2020/6688084 |
_version_ | 1783635892926152704 |
---|---|
author | Che Soh, Nor ‘Awatif Rapi, Hannah Syahirah Mohd Azam, Nurul Shahirah Santhanam, Ramesh Kumar Assaw, Suvik Haron, Mohd Nizam Ali, Abdul Manaf Maulidiani, M. Idris, Izwandy Ismail, Wan Iryani Wan |
author_facet | Che Soh, Nor ‘Awatif Rapi, Hannah Syahirah Mohd Azam, Nurul Shahirah Santhanam, Ramesh Kumar Assaw, Suvik Haron, Mohd Nizam Ali, Abdul Manaf Maulidiani, M. Idris, Izwandy Ismail, Wan Iryani Wan |
author_sort | Che Soh, Nor ‘Awatif |
collection | PubMed |
description | Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, the wound healing potential of D. claparedii aqueous extract on acute wound model in rats was revealed for the first time. Various concentrations (0.1%, 0.5%, and 1.0% w/w) of D. claparedii ointment were formulated and tested on Sprague Dawley rats through topical application on full-thickness skin wounds for 14 days. The wound healing effects were investigated via behaviour observation, wound contraction, and histopathological analysis. Quality assessment was performed via skin irritation test, microbial contamination test (MCT), and heavy metal detection. The study also included test for antibacterial activities and detection of bioactive compounds in D. claparedii. One percent of D. claparedii ointment showed rapid wound healing potential with good soothing effects and more collagen deposition in comparison to the commercial wound healing ointments such as acriflavine (0.1% w/v) and traditional ointment gamat (sea cucumber extract) (15.0% w/v). No local skin irritation, microbial contamination, and insignificant concentration of heavy metals were observed, which indicate its safe application. Moreover, the aqueous extract of D. claparedii exhibited antibacterial activities against Escherichia coli and Pseudomonas aeruginosa with minimum inhibitory concentration (MIC) value at 0.4 g/ml. (1)H NMR analysis of the aqueous extract of D. claparedii revealed some metabolites that might be responsible for its wound healing properties such as amino acids, halogenated aromatics, organic acids, vitamins, and others. Altogether, these results suggested that the aqueous extract of D. claparedii could be utilised as an alternative natural wound healing promoter. |
format | Online Article Text |
id | pubmed-7803155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-78031552021-01-22 Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats Che Soh, Nor ‘Awatif Rapi, Hannah Syahirah Mohd Azam, Nurul Shahirah Santhanam, Ramesh Kumar Assaw, Suvik Haron, Mohd Nizam Ali, Abdul Manaf Maulidiani, M. Idris, Izwandy Ismail, Wan Iryani Wan Evid Based Complement Alternat Med Research Article Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, the wound healing potential of D. claparedii aqueous extract on acute wound model in rats was revealed for the first time. Various concentrations (0.1%, 0.5%, and 1.0% w/w) of D. claparedii ointment were formulated and tested on Sprague Dawley rats through topical application on full-thickness skin wounds for 14 days. The wound healing effects were investigated via behaviour observation, wound contraction, and histopathological analysis. Quality assessment was performed via skin irritation test, microbial contamination test (MCT), and heavy metal detection. The study also included test for antibacterial activities and detection of bioactive compounds in D. claparedii. One percent of D. claparedii ointment showed rapid wound healing potential with good soothing effects and more collagen deposition in comparison to the commercial wound healing ointments such as acriflavine (0.1% w/v) and traditional ointment gamat (sea cucumber extract) (15.0% w/v). No local skin irritation, microbial contamination, and insignificant concentration of heavy metals were observed, which indicate its safe application. Moreover, the aqueous extract of D. claparedii exhibited antibacterial activities against Escherichia coli and Pseudomonas aeruginosa with minimum inhibitory concentration (MIC) value at 0.4 g/ml. (1)H NMR analysis of the aqueous extract of D. claparedii revealed some metabolites that might be responsible for its wound healing properties such as amino acids, halogenated aromatics, organic acids, vitamins, and others. Altogether, these results suggested that the aqueous extract of D. claparedii could be utilised as an alternative natural wound healing promoter. Hindawi 2020-12-28 /pmc/articles/PMC7803155/ /pubmed/33488747 http://dx.doi.org/10.1155/2020/6688084 Text en Copyright © 2020 Nor ‘Awatif Che Soh et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Che Soh, Nor ‘Awatif Rapi, Hannah Syahirah Mohd Azam, Nurul Shahirah Santhanam, Ramesh Kumar Assaw, Suvik Haron, Mohd Nizam Ali, Abdul Manaf Maulidiani, M. Idris, Izwandy Ismail, Wan Iryani Wan Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats |
title | Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats |
title_full | Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats |
title_fullStr | Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats |
title_full_unstemmed | Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats |
title_short | Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats |
title_sort | acute wound healing potential of marine worm, diopatra claparedii grube, 1878 aqueous extract on sprague dawley rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803155/ https://www.ncbi.nlm.nih.gov/pubmed/33488747 http://dx.doi.org/10.1155/2020/6688084 |
work_keys_str_mv | AT chesohnorawatif acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT rapihannahsyahirah acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT mohdazamnurulshahirah acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT santhanamrameshkumar acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT assawsuvik acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT haronmohdnizam acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT aliabdulmanaf acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT maulidianim acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT idrisizwandy acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats AT ismailwaniryaniwan acutewoundhealingpotentialofmarinewormdiopatraclaparediigrube1878aqueousextractonspraguedawleyrats |