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Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats

Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, th...

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Autores principales: Che Soh, Nor ‘Awatif, Rapi, Hannah Syahirah, Mohd Azam, Nurul Shahirah, Santhanam, Ramesh Kumar, Assaw, Suvik, Haron, Mohd Nizam, Ali, Abdul Manaf, Maulidiani, M., Idris, Izwandy, Ismail, Wan Iryani Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803155/
https://www.ncbi.nlm.nih.gov/pubmed/33488747
http://dx.doi.org/10.1155/2020/6688084
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author Che Soh, Nor ‘Awatif
Rapi, Hannah Syahirah
Mohd Azam, Nurul Shahirah
Santhanam, Ramesh Kumar
Assaw, Suvik
Haron, Mohd Nizam
Ali, Abdul Manaf
Maulidiani, M.
Idris, Izwandy
Ismail, Wan Iryani Wan
author_facet Che Soh, Nor ‘Awatif
Rapi, Hannah Syahirah
Mohd Azam, Nurul Shahirah
Santhanam, Ramesh Kumar
Assaw, Suvik
Haron, Mohd Nizam
Ali, Abdul Manaf
Maulidiani, M.
Idris, Izwandy
Ismail, Wan Iryani Wan
author_sort Che Soh, Nor ‘Awatif
collection PubMed
description Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, the wound healing potential of D. claparedii aqueous extract on acute wound model in rats was revealed for the first time. Various concentrations (0.1%, 0.5%, and 1.0% w/w) of D. claparedii ointment were formulated and tested on Sprague Dawley rats through topical application on full-thickness skin wounds for 14 days. The wound healing effects were investigated via behaviour observation, wound contraction, and histopathological analysis. Quality assessment was performed via skin irritation test, microbial contamination test (MCT), and heavy metal detection. The study also included test for antibacterial activities and detection of bioactive compounds in D. claparedii. One percent of D. claparedii ointment showed rapid wound healing potential with good soothing effects and more collagen deposition in comparison to the commercial wound healing ointments such as acriflavine (0.1% w/v) and traditional ointment gamat (sea cucumber extract) (15.0% w/v). No local skin irritation, microbial contamination, and insignificant concentration of heavy metals were observed, which indicate its safe application. Moreover, the aqueous extract of D. claparedii exhibited antibacterial activities against Escherichia coli and Pseudomonas aeruginosa with minimum inhibitory concentration (MIC) value at 0.4 g/ml. (1)H NMR analysis of the aqueous extract of D. claparedii revealed some metabolites that might be responsible for its wound healing properties such as amino acids, halogenated aromatics, organic acids, vitamins, and others. Altogether, these results suggested that the aqueous extract of D. claparedii could be utilised as an alternative natural wound healing promoter.
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spelling pubmed-78031552021-01-22 Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats Che Soh, Nor ‘Awatif Rapi, Hannah Syahirah Mohd Azam, Nurul Shahirah Santhanam, Ramesh Kumar Assaw, Suvik Haron, Mohd Nizam Ali, Abdul Manaf Maulidiani, M. Idris, Izwandy Ismail, Wan Iryani Wan Evid Based Complement Alternat Med Research Article Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, the wound healing potential of D. claparedii aqueous extract on acute wound model in rats was revealed for the first time. Various concentrations (0.1%, 0.5%, and 1.0% w/w) of D. claparedii ointment were formulated and tested on Sprague Dawley rats through topical application on full-thickness skin wounds for 14 days. The wound healing effects were investigated via behaviour observation, wound contraction, and histopathological analysis. Quality assessment was performed via skin irritation test, microbial contamination test (MCT), and heavy metal detection. The study also included test for antibacterial activities and detection of bioactive compounds in D. claparedii. One percent of D. claparedii ointment showed rapid wound healing potential with good soothing effects and more collagen deposition in comparison to the commercial wound healing ointments such as acriflavine (0.1% w/v) and traditional ointment gamat (sea cucumber extract) (15.0% w/v). No local skin irritation, microbial contamination, and insignificant concentration of heavy metals were observed, which indicate its safe application. Moreover, the aqueous extract of D. claparedii exhibited antibacterial activities against Escherichia coli and Pseudomonas aeruginosa with minimum inhibitory concentration (MIC) value at 0.4 g/ml. (1)H NMR analysis of the aqueous extract of D. claparedii revealed some metabolites that might be responsible for its wound healing properties such as amino acids, halogenated aromatics, organic acids, vitamins, and others. Altogether, these results suggested that the aqueous extract of D. claparedii could be utilised as an alternative natural wound healing promoter. Hindawi 2020-12-28 /pmc/articles/PMC7803155/ /pubmed/33488747 http://dx.doi.org/10.1155/2020/6688084 Text en Copyright © 2020 Nor ‘Awatif Che Soh et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Che Soh, Nor ‘Awatif
Rapi, Hannah Syahirah
Mohd Azam, Nurul Shahirah
Santhanam, Ramesh Kumar
Assaw, Suvik
Haron, Mohd Nizam
Ali, Abdul Manaf
Maulidiani, M.
Idris, Izwandy
Ismail, Wan Iryani Wan
Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats
title Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats
title_full Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats
title_fullStr Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats
title_full_unstemmed Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats
title_short Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats
title_sort acute wound healing potential of marine worm, diopatra claparedii grube, 1878 aqueous extract on sprague dawley rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803155/
https://www.ncbi.nlm.nih.gov/pubmed/33488747
http://dx.doi.org/10.1155/2020/6688084
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