Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli

Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication....

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Autores principales: Helgesen, Emily, Sætre, Frank, Skarstad, Kirsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803763/
https://www.ncbi.nlm.nih.gov/pubmed/33436807
http://dx.doi.org/10.1038/s41598-020-80043-4
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author Helgesen, Emily
Sætre, Frank
Skarstad, Kirsten
author_facet Helgesen, Emily
Sætre, Frank
Skarstad, Kirsten
author_sort Helgesen, Emily
collection PubMed
description Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10–20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell.
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spelling pubmed-78037632021-01-13 Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli Helgesen, Emily Sætre, Frank Skarstad, Kirsten Sci Rep Article Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10–20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell. Nature Publishing Group UK 2021-01-12 /pmc/articles/PMC7803763/ /pubmed/33436807 http://dx.doi.org/10.1038/s41598-020-80043-4 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Helgesen, Emily
Sætre, Frank
Skarstad, Kirsten
Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_full Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_fullStr Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_full_unstemmed Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_short Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_sort topoisomerase iv tracks behind the replication fork and the seqa complex during dna replication in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803763/
https://www.ncbi.nlm.nih.gov/pubmed/33436807
http://dx.doi.org/10.1038/s41598-020-80043-4
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