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Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening
The biological mechanisms involved in aseptic loosening include inflammation-associated and bone resorption-associated processes. Coordinated cellular actions result in biochemical imbalances with devastating consequences for the joint. Given that this condition is not known for showing systemic sig...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804331/ https://www.ncbi.nlm.nih.gov/pubmed/33436773 http://dx.doi.org/10.1038/s41598-020-79813-x |
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author | Vallés, Gema García-Rey, Eduardo Saldaña, Laura García-Cimbrelo, Eduardo Vilaboa, Nuria |
author_facet | Vallés, Gema García-Rey, Eduardo Saldaña, Laura García-Cimbrelo, Eduardo Vilaboa, Nuria |
author_sort | Vallés, Gema |
collection | PubMed |
description | The biological mechanisms involved in aseptic loosening include inflammation-associated and bone resorption-associated processes. Coordinated cellular actions result in biochemical imbalances with devastating consequences for the joint. Given that this condition is not known for showing systemic signs, we investigated whether circulating levels of inflammation-related proteins are altered in patients with aseptic loosening. Our study included 37 patients who underwent revision surgery due to hip osteolysis and aseptic loosening and 31 patients who underwent primary total hip arthroplasty. Using antibody arrays, we evaluated the serum levels of 320 proteins in four patients from each group. The results showed differences in insulin-like growth factor-binding protein 1 (IGFBP-1) concentrations, which we then quantified using enzyme-linked immunosorbent assay tests in all study patients. The results confirmed that serum IGFBP-1 concentrations were higher in the revision surgery patients than in the hip arthroplasty patients. In vitro studies showed that exposure of human osteoblasts to titanium particles induced an IGFBP-1 release that further increased when exposure to particles was performed in media conditioned by human M1 macrophages. These findings suggest that elevated serum IGFBP-1 levels in patients with aseptic loosening can arise from increased local IGFBP-1 production in the inflammatory environment of the periprosthetic bed. |
format | Online Article Text |
id | pubmed-7804331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78043312021-01-13 Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening Vallés, Gema García-Rey, Eduardo Saldaña, Laura García-Cimbrelo, Eduardo Vilaboa, Nuria Sci Rep Article The biological mechanisms involved in aseptic loosening include inflammation-associated and bone resorption-associated processes. Coordinated cellular actions result in biochemical imbalances with devastating consequences for the joint. Given that this condition is not known for showing systemic signs, we investigated whether circulating levels of inflammation-related proteins are altered in patients with aseptic loosening. Our study included 37 patients who underwent revision surgery due to hip osteolysis and aseptic loosening and 31 patients who underwent primary total hip arthroplasty. Using antibody arrays, we evaluated the serum levels of 320 proteins in four patients from each group. The results showed differences in insulin-like growth factor-binding protein 1 (IGFBP-1) concentrations, which we then quantified using enzyme-linked immunosorbent assay tests in all study patients. The results confirmed that serum IGFBP-1 concentrations were higher in the revision surgery patients than in the hip arthroplasty patients. In vitro studies showed that exposure of human osteoblasts to titanium particles induced an IGFBP-1 release that further increased when exposure to particles was performed in media conditioned by human M1 macrophages. These findings suggest that elevated serum IGFBP-1 levels in patients with aseptic loosening can arise from increased local IGFBP-1 production in the inflammatory environment of the periprosthetic bed. Nature Publishing Group UK 2021-01-12 /pmc/articles/PMC7804331/ /pubmed/33436773 http://dx.doi.org/10.1038/s41598-020-79813-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Vallés, Gema García-Rey, Eduardo Saldaña, Laura García-Cimbrelo, Eduardo Vilaboa, Nuria Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening |
title | Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening |
title_full | Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening |
title_fullStr | Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening |
title_full_unstemmed | Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening |
title_short | Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening |
title_sort | wear of hip prostheses increases serum igfbp-1 levels in patients with aseptic loosening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804331/ https://www.ncbi.nlm.nih.gov/pubmed/33436773 http://dx.doi.org/10.1038/s41598-020-79813-x |
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