Increasing AFM colloidal probe accuracy by optical tweezers

A precise determination of the cantilever spring constant is the critical point of all colloidal probe experiments. Existing methods are based on approximations considering only cantilever geometry and do not take into account properties of any object or substance attached to the cantilever. Neglect...

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Autores principales: Witko, Tomasz, Baster, Zbigniew, Rajfur, Zenon, Sofińska, Kamila, Barbasz, Jakub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804458/
https://www.ncbi.nlm.nih.gov/pubmed/33436725
http://dx.doi.org/10.1038/s41598-020-79938-z
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author Witko, Tomasz
Baster, Zbigniew
Rajfur, Zenon
Sofińska, Kamila
Barbasz, Jakub
author_facet Witko, Tomasz
Baster, Zbigniew
Rajfur, Zenon
Sofińska, Kamila
Barbasz, Jakub
author_sort Witko, Tomasz
collection PubMed
description A precise determination of the cantilever spring constant is the critical point of all colloidal probe experiments. Existing methods are based on approximations considering only cantilever geometry and do not take into account properties of any object or substance attached to the cantilever. Neglecting the influence of the colloidal sphere on the cantilever characteristics introduces significant uncertainty in a spring constant determination and affects all further considerations. In this work we propose a new method of spring constant calibration for ‘colloidal probe’ type cantilevers based on the direct measurement of force constant. The Optical Tweezers based calibration method will help to increase the accuracy and repeatability of the AFM colloidal probe experiments.
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spelling pubmed-78044582021-01-13 Increasing AFM colloidal probe accuracy by optical tweezers Witko, Tomasz Baster, Zbigniew Rajfur, Zenon Sofińska, Kamila Barbasz, Jakub Sci Rep Article A precise determination of the cantilever spring constant is the critical point of all colloidal probe experiments. Existing methods are based on approximations considering only cantilever geometry and do not take into account properties of any object or substance attached to the cantilever. Neglecting the influence of the colloidal sphere on the cantilever characteristics introduces significant uncertainty in a spring constant determination and affects all further considerations. In this work we propose a new method of spring constant calibration for ‘colloidal probe’ type cantilevers based on the direct measurement of force constant. The Optical Tweezers based calibration method will help to increase the accuracy and repeatability of the AFM colloidal probe experiments. Nature Publishing Group UK 2021-01-12 /pmc/articles/PMC7804458/ /pubmed/33436725 http://dx.doi.org/10.1038/s41598-020-79938-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Witko, Tomasz
Baster, Zbigniew
Rajfur, Zenon
Sofińska, Kamila
Barbasz, Jakub
Increasing AFM colloidal probe accuracy by optical tweezers
title Increasing AFM colloidal probe accuracy by optical tweezers
title_full Increasing AFM colloidal probe accuracy by optical tweezers
title_fullStr Increasing AFM colloidal probe accuracy by optical tweezers
title_full_unstemmed Increasing AFM colloidal probe accuracy by optical tweezers
title_short Increasing AFM colloidal probe accuracy by optical tweezers
title_sort increasing afm colloidal probe accuracy by optical tweezers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804458/
https://www.ncbi.nlm.nih.gov/pubmed/33436725
http://dx.doi.org/10.1038/s41598-020-79938-z
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