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Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense

The plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function...

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Autores principales: Cruz-Pérez, José Francisco, Lara-Oueilhe, Roxana, Marcos-Jiménez, Cynthia, Cuatlayotl-Olarte, Ricardo, Xiqui-Vázquez, María Luisa, Reyes-Carmona, Sandra Raquel, Baca, Beatriz Eugenia, Ramírez-Mata, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804937/
https://www.ncbi.nlm.nih.gov/pubmed/33436847
http://dx.doi.org/10.1038/s41598-020-80125-3
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author Cruz-Pérez, José Francisco
Lara-Oueilhe, Roxana
Marcos-Jiménez, Cynthia
Cuatlayotl-Olarte, Ricardo
Xiqui-Vázquez, María Luisa
Reyes-Carmona, Sandra Raquel
Baca, Beatriz Eugenia
Ramírez-Mata, Alberto
author_facet Cruz-Pérez, José Francisco
Lara-Oueilhe, Roxana
Marcos-Jiménez, Cynthia
Cuatlayotl-Olarte, Ricardo
Xiqui-Vázquez, María Luisa
Reyes-Carmona, Sandra Raquel
Baca, Beatriz Eugenia
Ramírez-Mata, Alberto
author_sort Cruz-Pérez, José Francisco
collection PubMed
description The plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function and expression of the cdgD gene, encoding a multidomain protein that includes GGDEF-EAL domains and CHASE and PAS domains. An insertional cdgD gene mutant was constructed, and analysis of biofilm and extracellular polymeric substance production, as well as the motility phenotype indicated that cdgD encoded a functional diguanylate protein. These results were correlated with a reduced overall cellular concentration of cyclic-di-GMP in the mutant over 48 h compared with that observed in the wild-type strain, which was recovered in the complemented strain. In addition, cdgD gene expression was measured in cells growing under planktonic or biofilm conditions, and differential expression was observed when KNO(3) or NH(4)Cl was added to the minimal medium as a nitrogen source. The transcriptional fusion of the cdgD promoter with the gene encoding the autofluorescent mCherry protein indicated that the cdgD gene was expressed both under abiotic conditions and in association with wheat roots. Reduced colonization of wheat roots was observed for the mutant compared with the wild-type strain grown in the same soil conditions. The Azospirillum-plant association begins with the motility of the bacterium towards the plant rhizosphere followed by the adsorption and adherence of these bacteria to plant roots. Therefore, it is important to study the genes that contribute to this initial interaction of the bacterium with its host plant.
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spelling pubmed-78049372021-01-13 Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense Cruz-Pérez, José Francisco Lara-Oueilhe, Roxana Marcos-Jiménez, Cynthia Cuatlayotl-Olarte, Ricardo Xiqui-Vázquez, María Luisa Reyes-Carmona, Sandra Raquel Baca, Beatriz Eugenia Ramírez-Mata, Alberto Sci Rep Article The plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function and expression of the cdgD gene, encoding a multidomain protein that includes GGDEF-EAL domains and CHASE and PAS domains. An insertional cdgD gene mutant was constructed, and analysis of biofilm and extracellular polymeric substance production, as well as the motility phenotype indicated that cdgD encoded a functional diguanylate protein. These results were correlated with a reduced overall cellular concentration of cyclic-di-GMP in the mutant over 48 h compared with that observed in the wild-type strain, which was recovered in the complemented strain. In addition, cdgD gene expression was measured in cells growing under planktonic or biofilm conditions, and differential expression was observed when KNO(3) or NH(4)Cl was added to the minimal medium as a nitrogen source. The transcriptional fusion of the cdgD promoter with the gene encoding the autofluorescent mCherry protein indicated that the cdgD gene was expressed both under abiotic conditions and in association with wheat roots. Reduced colonization of wheat roots was observed for the mutant compared with the wild-type strain grown in the same soil conditions. The Azospirillum-plant association begins with the motility of the bacterium towards the plant rhizosphere followed by the adsorption and adherence of these bacteria to plant roots. Therefore, it is important to study the genes that contribute to this initial interaction of the bacterium with its host plant. Nature Publishing Group UK 2021-01-12 /pmc/articles/PMC7804937/ /pubmed/33436847 http://dx.doi.org/10.1038/s41598-020-80125-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Cruz-Pérez, José Francisco
Lara-Oueilhe, Roxana
Marcos-Jiménez, Cynthia
Cuatlayotl-Olarte, Ricardo
Xiqui-Vázquez, María Luisa
Reyes-Carmona, Sandra Raquel
Baca, Beatriz Eugenia
Ramírez-Mata, Alberto
Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_full Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_fullStr Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_full_unstemmed Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_short Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_sort expression and function of the cdgd gene, encoding a chase–pas-dgc-eal domain protein, in azospirillum brasilense
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804937/
https://www.ncbi.nlm.nih.gov/pubmed/33436847
http://dx.doi.org/10.1038/s41598-020-80125-3
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