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The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohist...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804952/ https://www.ncbi.nlm.nih.gov/pubmed/33436714 http://dx.doi.org/10.1038/s41598-020-79780-3 |
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author | Okuma, Yu Aoki, Tomoaki Miyara, Santiago J. Hayashida, Kei Nishikimi, Mitsuaki Takegawa, Ryosuke Yin, Tai Kim, Junhwan Becker, Lance B. Shinozaki, Koichiro |
author_facet | Okuma, Yu Aoki, Tomoaki Miyara, Santiago J. Hayashida, Kei Nishikimi, Mitsuaki Takegawa, Ryosuke Yin, Tai Kim, Junhwan Becker, Lance B. Shinozaki, Koichiro |
author_sort | Okuma, Yu |
collection | PubMed |
description | The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohistochemistry and ELISA assays were used to evaluate the pituitary damage and endocrine function. Samples were collected at pre-CA, and 30 and 120 min after cardio pulmonary resuscitation. Triphenyltetrazolium chloride (TTC) staining demonstrated the expansion of the pituitary damage over time. There was phenotypic validity between the pars distalis and nervosa. Both CT-proAVP (pars nervosa hormone) and GH/IGF-1 (pars distalis hormone) decreased over time, and a different expression pattern corresponding to the damaged areas was noted (CT-proAVP, 30.2 ± 6.2, 31.5 ± 5.9, and 16.3 ± 7.6 pg/mg protein, p < 0.01; GH/IGF-1, 2.63 ± 0.61, 0.62 ± 0.36, and 2.01 ± 0.41 ng/mg protein, p < 0.01 respectively). Similarly, the expression pattern between these hormones in the end-organ systems showed phenotypic validity. Plasma CT-proAVP (r = 0.771, p = 0.025) and IGF-1 (r = −0.775, p = 0.024) demonstrated a strong correlation with TTC staining area. Our data suggested that CA induces pathological and functional damage to the pituitary gland. |
format | Online Article Text |
id | pubmed-7804952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78049522021-01-13 The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model Okuma, Yu Aoki, Tomoaki Miyara, Santiago J. Hayashida, Kei Nishikimi, Mitsuaki Takegawa, Ryosuke Yin, Tai Kim, Junhwan Becker, Lance B. Shinozaki, Koichiro Sci Rep Article The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohistochemistry and ELISA assays were used to evaluate the pituitary damage and endocrine function. Samples were collected at pre-CA, and 30 and 120 min after cardio pulmonary resuscitation. Triphenyltetrazolium chloride (TTC) staining demonstrated the expansion of the pituitary damage over time. There was phenotypic validity between the pars distalis and nervosa. Both CT-proAVP (pars nervosa hormone) and GH/IGF-1 (pars distalis hormone) decreased over time, and a different expression pattern corresponding to the damaged areas was noted (CT-proAVP, 30.2 ± 6.2, 31.5 ± 5.9, and 16.3 ± 7.6 pg/mg protein, p < 0.01; GH/IGF-1, 2.63 ± 0.61, 0.62 ± 0.36, and 2.01 ± 0.41 ng/mg protein, p < 0.01 respectively). Similarly, the expression pattern between these hormones in the end-organ systems showed phenotypic validity. Plasma CT-proAVP (r = 0.771, p = 0.025) and IGF-1 (r = −0.775, p = 0.024) demonstrated a strong correlation with TTC staining area. Our data suggested that CA induces pathological and functional damage to the pituitary gland. Nature Publishing Group UK 2021-01-12 /pmc/articles/PMC7804952/ /pubmed/33436714 http://dx.doi.org/10.1038/s41598-020-79780-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Okuma, Yu Aoki, Tomoaki Miyara, Santiago J. Hayashida, Kei Nishikimi, Mitsuaki Takegawa, Ryosuke Yin, Tai Kim, Junhwan Becker, Lance B. Shinozaki, Koichiro The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
title | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
title_full | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
title_fullStr | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
title_full_unstemmed | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
title_short | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
title_sort | evaluation of pituitary damage associated with cardiac arrest: an experimental rodent model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804952/ https://www.ncbi.nlm.nih.gov/pubmed/33436714 http://dx.doi.org/10.1038/s41598-020-79780-3 |
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