Cargando…

A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke

Background: We aimed to develop a diagnostic indicator of stroke based on serum miRNAs correlated to systolic blood pressure. Methods: Using miRNA expression profiles in GSE117604 from the Gene Expression Omnibus (GEO), we utilized the WGCNA to identify hub miRNAs correlated to systolic blood pressu...

Descripción completa

Detalles Bibliográficos
Autores principales: Qi, Yujun, Yuan, Mingfeng, Yi, Qiong, Wang, Yan, Xu, Lei, Xu, Changsong, Lu, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805026/
https://www.ncbi.nlm.nih.gov/pubmed/33345284
http://dx.doi.org/10.1042/BSR20203458
_version_ 1783636234661265408
author Qi, Yujun
Yuan, Mingfeng
Yi, Qiong
Wang, Yan
Xu, Lei
Xu, Changsong
Lu, Min
author_facet Qi, Yujun
Yuan, Mingfeng
Yi, Qiong
Wang, Yan
Xu, Lei
Xu, Changsong
Lu, Min
author_sort Qi, Yujun
collection PubMed
description Background: We aimed to develop a diagnostic indicator of stroke based on serum miRNAs correlated to systolic blood pressure. Methods: Using miRNA expression profiles in GSE117604 from the Gene Expression Omnibus (GEO), we utilized the WGCNA to identify hub miRNAs correlated to systolic blood pressure (SBP). Differential analysis was applied to highlight hub differentially expressed miRNAs (DE-miRNAs), whereby we built a miRNA-based diagnostic indicator for stroke using bootstrap ranking Least Absolute Shrinkage and Selection Operator (LASSO) regression with 10-fold cross-validation. The classification value of the indicator was validated with receiver operating characteristic (ROC) analysis in both the training set and test set, as well as quantitative real-time PCR (qRT-PCR) for the feature miRNAs. Further, target genes of hub miRNAs and hub DE-miRNAs were retrieved for functional enrichment. Results: A total of 447 hub miRNAs in the blue modules were significantly correlated with systolic blood pressure (r = 0.32, false discovery rate = 10(−6)). Target genes predicted with the hub miRNAs were mostly implicated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) terms including mitogen-activated protein kinase (MAPK) pathway, senescence, and TGF-β signaling pathway. The diagnostic indicator with miR-4420 and miR-6793-5p showed remarkable performance in the training set (area under curve [AUC]= 0.953), as well as in the test set (AUC = 0.894). Results of qRT-PCR validated the diagnostic value of the two miRNAs embedded in the proposed indicator. Conclusions: We developed a panel of two miRNAs, which is a good diagnostic indicator for stroke. These results require further investigation.
format Online
Article
Text
id pubmed-7805026
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-78050262021-01-25 A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke Qi, Yujun Yuan, Mingfeng Yi, Qiong Wang, Yan Xu, Lei Xu, Changsong Lu, Min Biosci Rep Bioinformatics Background: We aimed to develop a diagnostic indicator of stroke based on serum miRNAs correlated to systolic blood pressure. Methods: Using miRNA expression profiles in GSE117604 from the Gene Expression Omnibus (GEO), we utilized the WGCNA to identify hub miRNAs correlated to systolic blood pressure (SBP). Differential analysis was applied to highlight hub differentially expressed miRNAs (DE-miRNAs), whereby we built a miRNA-based diagnostic indicator for stroke using bootstrap ranking Least Absolute Shrinkage and Selection Operator (LASSO) regression with 10-fold cross-validation. The classification value of the indicator was validated with receiver operating characteristic (ROC) analysis in both the training set and test set, as well as quantitative real-time PCR (qRT-PCR) for the feature miRNAs. Further, target genes of hub miRNAs and hub DE-miRNAs were retrieved for functional enrichment. Results: A total of 447 hub miRNAs in the blue modules were significantly correlated with systolic blood pressure (r = 0.32, false discovery rate = 10(−6)). Target genes predicted with the hub miRNAs were mostly implicated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) terms including mitogen-activated protein kinase (MAPK) pathway, senescence, and TGF-β signaling pathway. The diagnostic indicator with miR-4420 and miR-6793-5p showed remarkable performance in the training set (area under curve [AUC]= 0.953), as well as in the test set (AUC = 0.894). Results of qRT-PCR validated the diagnostic value of the two miRNAs embedded in the proposed indicator. Conclusions: We developed a panel of two miRNAs, which is a good diagnostic indicator for stroke. These results require further investigation. Portland Press Ltd. 2021-01-12 /pmc/articles/PMC7805026/ /pubmed/33345284 http://dx.doi.org/10.1042/BSR20203458 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bioinformatics
Qi, Yujun
Yuan, Mingfeng
Yi, Qiong
Wang, Yan
Xu, Lei
Xu, Changsong
Lu, Min
A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke
title A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke
title_full A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke
title_fullStr A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke
title_full_unstemmed A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke
title_short A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke
title_sort panel of two mirnas correlated to systolic blood pressure is a good diagnostic indicator for stroke
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805026/
https://www.ncbi.nlm.nih.gov/pubmed/33345284
http://dx.doi.org/10.1042/BSR20203458
work_keys_str_mv AT qiyujun apaneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT yuanmingfeng apaneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT yiqiong apaneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT wangyan apaneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT xulei apaneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT xuchangsong apaneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT lumin apaneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT qiyujun paneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT yuanmingfeng paneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT yiqiong paneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT wangyan paneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT xulei paneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT xuchangsong paneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke
AT lumin paneloftwomirnascorrelatedtosystolicbloodpressureisagooddiagnosticindicatorforstroke