Cargando…

Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations

BACKGROUND: Characterizing the genetic diversity of malaria parasite populations in different endemic settings (from low to high) could be helpful in determining the effectiveness of malaria interventions. This study compared Plasmodium falciparum parasite population diversity from two sites with lo...

Descripción completa

Detalles Bibliográficos
Autores principales: Oboh, Mary A., Ndiaye, Tolla, Diongue, Khadim, Ndiaye, Yaye D., Sy, Mouhamad, Deme, Awa B., Diallo, Mamadou A., Yade, Mamadou S., Volkman, Sarah K., Badiane, Aida S., Amambua-Ngwa, Alfred, Ndiaye, Daouda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805152/
https://www.ncbi.nlm.nih.gov/pubmed/33436004
http://dx.doi.org/10.1186/s12936-020-03563-4
_version_ 1783636260808556544
author Oboh, Mary A.
Ndiaye, Tolla
Diongue, Khadim
Ndiaye, Yaye D.
Sy, Mouhamad
Deme, Awa B.
Diallo, Mamadou A.
Yade, Mamadou S.
Volkman, Sarah K.
Badiane, Aida S.
Amambua-Ngwa, Alfred
Ndiaye, Daouda
author_facet Oboh, Mary A.
Ndiaye, Tolla
Diongue, Khadim
Ndiaye, Yaye D.
Sy, Mouhamad
Deme, Awa B.
Diallo, Mamadou A.
Yade, Mamadou S.
Volkman, Sarah K.
Badiane, Aida S.
Amambua-Ngwa, Alfred
Ndiaye, Daouda
author_sort Oboh, Mary A.
collection PubMed
description BACKGROUND: Characterizing the genetic diversity of malaria parasite populations in different endemic settings (from low to high) could be helpful in determining the effectiveness of malaria interventions. This study compared Plasmodium falciparum parasite population diversity from two sites with low (pre-elimination) and high transmission in Senegal and Nigeria, respectively. METHODS: Parasite genomic DNA was extracted from 187 dried blood spot collected from confirmed uncomplicated P. falciparum malaria infected patients in Senegal (94) and Nigeria (93). Allelic polymorphism at merozoite surface protein 1 (msp1) and merozoite surface protein- 2 (msp2) genes were assessed by nested PCR. RESULTS: The most frequent msp1 and msp2 allelic families are the K1 and IC3D7 allelotypes in both Senegal and Nigeria. Multiplicity of infection (MOI) of greater that 1 and thus complex infections was common in both study sites in Senegal (Thies:1.51/2.53; Kedougou:2.2/2.0 for msp1/2) than in Nigeria (Gbagada: 1.39/1.96; Oredo: 1.35/1.75]). The heterozygosity of msp1 gene was higher in P. falciparum isolates from Senegal (Thies: 0.62; Kedougou: 0.53) than isolates from Nigeria (Gbagada: 0.55; Oredo: 0.50). In Senegal, K1 alleles was associated with heavy than with moderate parasite density. Meanwhile, equal proportions of K1 were observed in both heavy and moderate infection types in Nigeria. The IC3D7 subtype allele of the msp2 family was the most frequent in heavily parasitaemic individuals from both countries than in the moderately infected participants. CONCLUSION: The unexpectedly low genetic diversity of infections high endemic Nigerian setting compared to the low endemic settings in Senegal is suggestive of possible epidemic outbreak in Nigeria.
format Online
Article
Text
id pubmed-7805152
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-78051522021-01-14 Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations Oboh, Mary A. Ndiaye, Tolla Diongue, Khadim Ndiaye, Yaye D. Sy, Mouhamad Deme, Awa B. Diallo, Mamadou A. Yade, Mamadou S. Volkman, Sarah K. Badiane, Aida S. Amambua-Ngwa, Alfred Ndiaye, Daouda Malar J Research BACKGROUND: Characterizing the genetic diversity of malaria parasite populations in different endemic settings (from low to high) could be helpful in determining the effectiveness of malaria interventions. This study compared Plasmodium falciparum parasite population diversity from two sites with low (pre-elimination) and high transmission in Senegal and Nigeria, respectively. METHODS: Parasite genomic DNA was extracted from 187 dried blood spot collected from confirmed uncomplicated P. falciparum malaria infected patients in Senegal (94) and Nigeria (93). Allelic polymorphism at merozoite surface protein 1 (msp1) and merozoite surface protein- 2 (msp2) genes were assessed by nested PCR. RESULTS: The most frequent msp1 and msp2 allelic families are the K1 and IC3D7 allelotypes in both Senegal and Nigeria. Multiplicity of infection (MOI) of greater that 1 and thus complex infections was common in both study sites in Senegal (Thies:1.51/2.53; Kedougou:2.2/2.0 for msp1/2) than in Nigeria (Gbagada: 1.39/1.96; Oredo: 1.35/1.75]). The heterozygosity of msp1 gene was higher in P. falciparum isolates from Senegal (Thies: 0.62; Kedougou: 0.53) than isolates from Nigeria (Gbagada: 0.55; Oredo: 0.50). In Senegal, K1 alleles was associated with heavy than with moderate parasite density. Meanwhile, equal proportions of K1 were observed in both heavy and moderate infection types in Nigeria. The IC3D7 subtype allele of the msp2 family was the most frequent in heavily parasitaemic individuals from both countries than in the moderately infected participants. CONCLUSION: The unexpectedly low genetic diversity of infections high endemic Nigerian setting compared to the low endemic settings in Senegal is suggestive of possible epidemic outbreak in Nigeria. BioMed Central 2021-01-13 /pmc/articles/PMC7805152/ /pubmed/33436004 http://dx.doi.org/10.1186/s12936-020-03563-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Oboh, Mary A.
Ndiaye, Tolla
Diongue, Khadim
Ndiaye, Yaye D.
Sy, Mouhamad
Deme, Awa B.
Diallo, Mamadou A.
Yade, Mamadou S.
Volkman, Sarah K.
Badiane, Aida S.
Amambua-Ngwa, Alfred
Ndiaye, Daouda
Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
title Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
title_full Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
title_fullStr Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
title_full_unstemmed Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
title_short Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
title_sort allelic diversity of msp1 and msp2 repeat loci correlate with levels of malaria endemicity in senegal and nigerian populations
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805152/
https://www.ncbi.nlm.nih.gov/pubmed/33436004
http://dx.doi.org/10.1186/s12936-020-03563-4
work_keys_str_mv AT obohmarya allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT ndiayetolla allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT dionguekhadim allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT ndiayeyayed allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT symouhamad allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT demeawab allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT diallomamadoua allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT yademamadous allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT volkmansarahk allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT badianeaidas allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT amambuangwaalfred allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations
AT ndiayedaouda allelicdiversityofmsp1andmsp2repeatlocicorrelatewithlevelsofmalariaendemicityinsenegalandnigerianpopulations