The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex

Dendritic actin networks develop from a first actin filament through branching by the Arp2/3 complex. At the surface of endosomes, the WASH complex activates the Arp2/3 complex and interacts with the capping protein for unclear reasons. Here, we show that the WASH complex interacts with dynactin and...

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Autores principales: Fokin, Artem I., David, Violaine, Oguievetskaia, Ksenia, Derivery, Emmanuel, Stone, Caroline E., Cao, Luyan, Rocques, Nathalie, Molinie, Nicolas, Henriot, Véronique, Aumont-Nicaise, Magali, Hinckelmann, Maria-Victoria, Saudou, Frédéric, Le Clainche, Christophe, Carter, Andrew P., Romet-Lemonne, Guillaume, Gautreau, Alexis M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806238/
https://www.ncbi.nlm.nih.gov/pubmed/33523880
http://dx.doi.org/10.1126/sciadv.abd5956
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author Fokin, Artem I.
David, Violaine
Oguievetskaia, Ksenia
Derivery, Emmanuel
Stone, Caroline E.
Cao, Luyan
Rocques, Nathalie
Molinie, Nicolas
Henriot, Véronique
Aumont-Nicaise, Magali
Hinckelmann, Maria-Victoria
Saudou, Frédéric
Le Clainche, Christophe
Carter, Andrew P.
Romet-Lemonne, Guillaume
Gautreau, Alexis M.
author_facet Fokin, Artem I.
David, Violaine
Oguievetskaia, Ksenia
Derivery, Emmanuel
Stone, Caroline E.
Cao, Luyan
Rocques, Nathalie
Molinie, Nicolas
Henriot, Véronique
Aumont-Nicaise, Magali
Hinckelmann, Maria-Victoria
Saudou, Frédéric
Le Clainche, Christophe
Carter, Andrew P.
Romet-Lemonne, Guillaume
Gautreau, Alexis M.
author_sort Fokin, Artem I.
collection PubMed
description Dendritic actin networks develop from a first actin filament through branching by the Arp2/3 complex. At the surface of endosomes, the WASH complex activates the Arp2/3 complex and interacts with the capping protein for unclear reasons. Here, we show that the WASH complex interacts with dynactin and uncaps it through its FAM21 subunit. In vitro, the uncapped Arp1/11 minifilament elongates an actin filament, which then primes the WASH-induced Arp2/3 branching reaction. In dynactin-depleted cells or in cells where the WASH complex is reconstituted with a FAM21 mutant that cannot uncap dynactin, formation of branched actin at the endosomal surface is impaired. Our results reveal the importance of the WASH complex in coordinating two complexes containing actin-related proteins.
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spelling pubmed-78062382021-01-21 The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex Fokin, Artem I. David, Violaine Oguievetskaia, Ksenia Derivery, Emmanuel Stone, Caroline E. Cao, Luyan Rocques, Nathalie Molinie, Nicolas Henriot, Véronique Aumont-Nicaise, Magali Hinckelmann, Maria-Victoria Saudou, Frédéric Le Clainche, Christophe Carter, Andrew P. Romet-Lemonne, Guillaume Gautreau, Alexis M. Sci Adv Research Articles Dendritic actin networks develop from a first actin filament through branching by the Arp2/3 complex. At the surface of endosomes, the WASH complex activates the Arp2/3 complex and interacts with the capping protein for unclear reasons. Here, we show that the WASH complex interacts with dynactin and uncaps it through its FAM21 subunit. In vitro, the uncapped Arp1/11 minifilament elongates an actin filament, which then primes the WASH-induced Arp2/3 branching reaction. In dynactin-depleted cells or in cells where the WASH complex is reconstituted with a FAM21 mutant that cannot uncap dynactin, formation of branched actin at the endosomal surface is impaired. Our results reveal the importance of the WASH complex in coordinating two complexes containing actin-related proteins. American Association for the Advancement of Science 2021-01-13 /pmc/articles/PMC7806238/ /pubmed/33523880 http://dx.doi.org/10.1126/sciadv.abd5956 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Fokin, Artem I.
David, Violaine
Oguievetskaia, Ksenia
Derivery, Emmanuel
Stone, Caroline E.
Cao, Luyan
Rocques, Nathalie
Molinie, Nicolas
Henriot, Véronique
Aumont-Nicaise, Magali
Hinckelmann, Maria-Victoria
Saudou, Frédéric
Le Clainche, Christophe
Carter, Andrew P.
Romet-Lemonne, Guillaume
Gautreau, Alexis M.
The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex
title The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex
title_full The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex
title_fullStr The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex
title_full_unstemmed The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex
title_short The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex
title_sort arp1/11 minifilament of dynactin primes the endosomal arp2/3 complex
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806238/
https://www.ncbi.nlm.nih.gov/pubmed/33523880
http://dx.doi.org/10.1126/sciadv.abd5956
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