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Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase
Checkpoints maintain the order of cell cycle events during DNA damage or incomplete replication. How the checkpoint response is tailored to different phases of the cell cycle remains poorly understood. The S-phase checkpoint for example results in the slowing of replication, which in budding yeast o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806266/ https://www.ncbi.nlm.nih.gov/pubmed/33399537 http://dx.doi.org/10.7554/eLife.63589 |
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author | Johnson, Mark C Can, Geylani Santos, Miguel Monteiro Alexander, Diana Zegerman, Philip |
author_facet | Johnson, Mark C Can, Geylani Santos, Miguel Monteiro Alexander, Diana Zegerman, Philip |
author_sort | Johnson, Mark C |
collection | PubMed |
description | Checkpoints maintain the order of cell cycle events during DNA damage or incomplete replication. How the checkpoint response is tailored to different phases of the cell cycle remains poorly understood. The S-phase checkpoint for example results in the slowing of replication, which in budding yeast occurs by Rad53-dependent inhibition of the initiation factors Sld3 and Dbf4. Despite this, we show here that Rad53 phosphorylates both of these substrates throughout the cell cycle at the same sites as in S-phase, suggesting roles for this pathway beyond S-phase. Indeed, we show that Rad53-dependent inhibition of Sld3 and Dbf4 limits re-replication in G2/M, preventing gene amplification. In addition, we show that inhibition of Sld3 and Dbf4 in G1 prevents premature initiation at all origins at the G1/S transition. This study redefines the scope of the ‘S-phase checkpoint’ with implications for understanding checkpoint function in cancers that lack cell cycle controls. |
format | Online Article Text |
id | pubmed-7806266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-78062662021-01-15 Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase Johnson, Mark C Can, Geylani Santos, Miguel Monteiro Alexander, Diana Zegerman, Philip eLife Chromosomes and Gene Expression Checkpoints maintain the order of cell cycle events during DNA damage or incomplete replication. How the checkpoint response is tailored to different phases of the cell cycle remains poorly understood. The S-phase checkpoint for example results in the slowing of replication, which in budding yeast occurs by Rad53-dependent inhibition of the initiation factors Sld3 and Dbf4. Despite this, we show here that Rad53 phosphorylates both of these substrates throughout the cell cycle at the same sites as in S-phase, suggesting roles for this pathway beyond S-phase. Indeed, we show that Rad53-dependent inhibition of Sld3 and Dbf4 limits re-replication in G2/M, preventing gene amplification. In addition, we show that inhibition of Sld3 and Dbf4 in G1 prevents premature initiation at all origins at the G1/S transition. This study redefines the scope of the ‘S-phase checkpoint’ with implications for understanding checkpoint function in cancers that lack cell cycle controls. eLife Sciences Publications, Ltd 2021-01-05 /pmc/articles/PMC7806266/ /pubmed/33399537 http://dx.doi.org/10.7554/eLife.63589 Text en © 2021, Johnson et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Chromosomes and Gene Expression Johnson, Mark C Can, Geylani Santos, Miguel Monteiro Alexander, Diana Zegerman, Philip Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase |
title | Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase |
title_full | Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase |
title_fullStr | Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase |
title_full_unstemmed | Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase |
title_short | Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase |
title_sort | checkpoint inhibition of origin firing prevents inappropriate replication outside of s-phase |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806266/ https://www.ncbi.nlm.nih.gov/pubmed/33399537 http://dx.doi.org/10.7554/eLife.63589 |
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