Cargando…
Dentate gyrus development requires a cortical hem-derived astrocytic scaffold
During embryonic development, radial glial cells give rise to neurons, then to astrocytes following the gliogenic switch. Timely regulation of the switch, operated by several transcription factors, is fundamental for allowing coordinated interactions between neurons and glia. We deleted the gene for...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806271/ https://www.ncbi.nlm.nih.gov/pubmed/33393905 http://dx.doi.org/10.7554/eLife.63904 |
_version_ | 1783636493206552576 |
---|---|
author | Caramello, Alessia Galichet, Christophe Rizzoti, Karine Lovell-Badge, Robin |
author_facet | Caramello, Alessia Galichet, Christophe Rizzoti, Karine Lovell-Badge, Robin |
author_sort | Caramello, Alessia |
collection | PubMed |
description | During embryonic development, radial glial cells give rise to neurons, then to astrocytes following the gliogenic switch. Timely regulation of the switch, operated by several transcription factors, is fundamental for allowing coordinated interactions between neurons and glia. We deleted the gene for one such factor, SOX9, early during mouse brain development and observed a significantly compromised dentate gyrus (DG). We dissected the origin of the defect, targeting embryonic Sox9 deletion to either the DG neuronal progenitor domain or the adjacent cortical hem (CH). We identified in the latter previously uncharacterized ALDH1L1+ astrocytic progenitors, which form a fimbrial-specific glial scaffold necessary for neuronal progenitor migration toward the developing DG. Our results highlight an early crucial role of SOX9 for DG development through regulation of astroglial potential acquisition in the CH. Moreover, we illustrate how formation of a local network, amidst astrocytic and neuronal progenitors originating from adjacent domains, underlays brain morphogenesis. |
format | Online Article Text |
id | pubmed-7806271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-78062712021-01-15 Dentate gyrus development requires a cortical hem-derived astrocytic scaffold Caramello, Alessia Galichet, Christophe Rizzoti, Karine Lovell-Badge, Robin eLife Developmental Biology During embryonic development, radial glial cells give rise to neurons, then to astrocytes following the gliogenic switch. Timely regulation of the switch, operated by several transcription factors, is fundamental for allowing coordinated interactions between neurons and glia. We deleted the gene for one such factor, SOX9, early during mouse brain development and observed a significantly compromised dentate gyrus (DG). We dissected the origin of the defect, targeting embryonic Sox9 deletion to either the DG neuronal progenitor domain or the adjacent cortical hem (CH). We identified in the latter previously uncharacterized ALDH1L1+ astrocytic progenitors, which form a fimbrial-specific glial scaffold necessary for neuronal progenitor migration toward the developing DG. Our results highlight an early crucial role of SOX9 for DG development through regulation of astroglial potential acquisition in the CH. Moreover, we illustrate how formation of a local network, amidst astrocytic and neuronal progenitors originating from adjacent domains, underlays brain morphogenesis. eLife Sciences Publications, Ltd 2021-01-04 /pmc/articles/PMC7806271/ /pubmed/33393905 http://dx.doi.org/10.7554/eLife.63904 Text en © 2021, Caramello et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Caramello, Alessia Galichet, Christophe Rizzoti, Karine Lovell-Badge, Robin Dentate gyrus development requires a cortical hem-derived astrocytic scaffold |
title | Dentate gyrus development requires a cortical hem-derived astrocytic scaffold |
title_full | Dentate gyrus development requires a cortical hem-derived astrocytic scaffold |
title_fullStr | Dentate gyrus development requires a cortical hem-derived astrocytic scaffold |
title_full_unstemmed | Dentate gyrus development requires a cortical hem-derived astrocytic scaffold |
title_short | Dentate gyrus development requires a cortical hem-derived astrocytic scaffold |
title_sort | dentate gyrus development requires a cortical hem-derived astrocytic scaffold |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806271/ https://www.ncbi.nlm.nih.gov/pubmed/33393905 http://dx.doi.org/10.7554/eLife.63904 |
work_keys_str_mv | AT caramelloalessia dentategyrusdevelopmentrequiresacorticalhemderivedastrocyticscaffold AT galichetchristophe dentategyrusdevelopmentrequiresacorticalhemderivedastrocyticscaffold AT rizzotikarine dentategyrusdevelopmentrequiresacorticalhemderivedastrocyticscaffold AT lovellbadgerobin dentategyrusdevelopmentrequiresacorticalhemderivedastrocyticscaffold |