Isolation of tumor-resident CD8(+) T cells from human lung tumors

CD103(+)CD8(+) tumor-resident memory T cells (T(RM)) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8(+) T cells and autologous tumor cells from human lung tumors to study the f...

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Autores principales: Corgnac, Stéphanie, Lecluse, Yann, Mami-chouaib, Fathia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806513/
https://www.ncbi.nlm.nih.gov/pubmed/33490983
http://dx.doi.org/10.1016/j.xpro.2020.100267
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author Corgnac, Stéphanie
Lecluse, Yann
Mami-chouaib, Fathia
author_facet Corgnac, Stéphanie
Lecluse, Yann
Mami-chouaib, Fathia
author_sort Corgnac, Stéphanie
collection PubMed
description CD103(+)CD8(+) tumor-resident memory T cells (T(RM)) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8(+) T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8(+) T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of T(RM) cells depends on the features of the tumor. For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020).
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spelling pubmed-78065132021-01-22 Isolation of tumor-resident CD8(+) T cells from human lung tumors Corgnac, Stéphanie Lecluse, Yann Mami-chouaib, Fathia STAR Protoc Protocol CD103(+)CD8(+) tumor-resident memory T cells (T(RM)) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8(+) T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8(+) T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of T(RM) cells depends on the features of the tumor. For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020). Elsevier 2021-01-12 /pmc/articles/PMC7806513/ /pubmed/33490983 http://dx.doi.org/10.1016/j.xpro.2020.100267 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Corgnac, Stéphanie
Lecluse, Yann
Mami-chouaib, Fathia
Isolation of tumor-resident CD8(+) T cells from human lung tumors
title Isolation of tumor-resident CD8(+) T cells from human lung tumors
title_full Isolation of tumor-resident CD8(+) T cells from human lung tumors
title_fullStr Isolation of tumor-resident CD8(+) T cells from human lung tumors
title_full_unstemmed Isolation of tumor-resident CD8(+) T cells from human lung tumors
title_short Isolation of tumor-resident CD8(+) T cells from human lung tumors
title_sort isolation of tumor-resident cd8(+) t cells from human lung tumors
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806513/
https://www.ncbi.nlm.nih.gov/pubmed/33490983
http://dx.doi.org/10.1016/j.xpro.2020.100267
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