Flow cytometry-based ex vivo murine NK cell cytotoxicity assay

Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium rele...

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Detalles Bibliográficos
Autores principales: Wong, Pamela, Wagner, Julia A., Berrien-Elliott, Melissa M., Schappe, Timothy, Fehniger, Todd A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806516/
https://www.ncbi.nlm.nih.gov/pubmed/33490978
http://dx.doi.org/10.1016/j.xpro.2020.100262
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author Wong, Pamela
Wagner, Julia A.
Berrien-Elliott, Melissa M.
Schappe, Timothy
Fehniger, Todd A.
author_facet Wong, Pamela
Wagner, Julia A.
Berrien-Elliott, Melissa M.
Schappe, Timothy
Fehniger, Todd A.
author_sort Wong, Pamela
collection PubMed
description Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol details specifically an ex vivo cytotoxicity assay using primary murine NK cells enriched from splenocytes that were activated in vivo with poly(I:C). For complete details on the use and execution of this protocol, please refer to Wagner et al. (2020).
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spelling pubmed-78065162021-01-22 Flow cytometry-based ex vivo murine NK cell cytotoxicity assay Wong, Pamela Wagner, Julia A. Berrien-Elliott, Melissa M. Schappe, Timothy Fehniger, Todd A. STAR Protoc Protocol Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol details specifically an ex vivo cytotoxicity assay using primary murine NK cells enriched from splenocytes that were activated in vivo with poly(I:C). For complete details on the use and execution of this protocol, please refer to Wagner et al. (2020). Elsevier 2021-01-12 /pmc/articles/PMC7806516/ /pubmed/33490978 http://dx.doi.org/10.1016/j.xpro.2020.100262 Text en © 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wong, Pamela
Wagner, Julia A.
Berrien-Elliott, Melissa M.
Schappe, Timothy
Fehniger, Todd A.
Flow cytometry-based ex vivo murine NK cell cytotoxicity assay
title Flow cytometry-based ex vivo murine NK cell cytotoxicity assay
title_full Flow cytometry-based ex vivo murine NK cell cytotoxicity assay
title_fullStr Flow cytometry-based ex vivo murine NK cell cytotoxicity assay
title_full_unstemmed Flow cytometry-based ex vivo murine NK cell cytotoxicity assay
title_short Flow cytometry-based ex vivo murine NK cell cytotoxicity assay
title_sort flow cytometry-based ex vivo murine nk cell cytotoxicity assay
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806516/
https://www.ncbi.nlm.nih.gov/pubmed/33490978
http://dx.doi.org/10.1016/j.xpro.2020.100262
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AT schappetimothy flowcytometrybasedexvivomurinenkcellcytotoxicityassay
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