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Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression

The tick Rhipicephalus microplus is a harmful parasite of cattle that causes considerable economic losses to the cattle breeding industry. Although R. microplus saliva (Rm-saliva) contains several immunosuppressants, any association between Rm-saliva and the expression of immunoinhibitory molecules,...

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Autores principales: Sajiki, Yamato, Konnai, Satoru, Ikenaka, Yoshinori, Gulay, Kevin Christian Montecillo, Kobayashi, Atsushi, Parizi, Luís Fernando, João, Benvindo Capela, Watari, Kei, Fujisawa, Sotaro, Okagawa, Tomohiro, Maekawa, Naoya, Logullo, Carlos, da Silva Vaz, Itabajara, Murata, Shiro, Ohashi, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806669/
https://www.ncbi.nlm.nih.gov/pubmed/33441793
http://dx.doi.org/10.1038/s41598-020-80251-y
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author Sajiki, Yamato
Konnai, Satoru
Ikenaka, Yoshinori
Gulay, Kevin Christian Montecillo
Kobayashi, Atsushi
Parizi, Luís Fernando
João, Benvindo Capela
Watari, Kei
Fujisawa, Sotaro
Okagawa, Tomohiro
Maekawa, Naoya
Logullo, Carlos
da Silva Vaz, Itabajara
Murata, Shiro
Ohashi, Kazuhiko
author_facet Sajiki, Yamato
Konnai, Satoru
Ikenaka, Yoshinori
Gulay, Kevin Christian Montecillo
Kobayashi, Atsushi
Parizi, Luís Fernando
João, Benvindo Capela
Watari, Kei
Fujisawa, Sotaro
Okagawa, Tomohiro
Maekawa, Naoya
Logullo, Carlos
da Silva Vaz, Itabajara
Murata, Shiro
Ohashi, Kazuhiko
author_sort Sajiki, Yamato
collection PubMed
description The tick Rhipicephalus microplus is a harmful parasite of cattle that causes considerable economic losses to the cattle breeding industry. Although R. microplus saliva (Rm-saliva) contains several immunosuppressants, any association between Rm-saliva and the expression of immunoinhibitory molecules, such as programmed death (PD)-1 and PD-ligand 1 (PD-L1), has not been described. In this study, flow cytometric analyses revealed that Rm-saliva upregulated PD-1 expression in T cells and PD-L1 expression in CD14(+) and CD11c(+) cells in cattle. Additionally, Rm-saliva decreased CD69 expression in T cells and Th1 cytokine production from peripheral blood mononuclear cells. Furthermore, PD-L1 blockade increased IFN-γ production in the presence of Rm-saliva, suggesting that Rm-saliva suppresses Th1 responses via the PD-1/PD-L1 pathway. To reveal the upregulation mechanism of PD-1/PD-L1 by Rm-saliva, we analyzed the function of prostaglandin E(2) (PGE(2)), which is known as an inducer of PD-L1 expression, in Rm-saliva. We found that Rm-saliva contained a high concentration of PGE(2), and PGE(2) treatment induced PD-L1 expression in CD14(+) cells in vitro. Immunohistochemical analyses revealed that PGE(2) and PD-L1 expression was upregulated in tick-attached skin in cattle. These data suggest that PGE(2) in Rm-saliva has the potential to induce the expression of immunoinhibitory molecules in host immune cells.
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spelling pubmed-78066692021-01-14 Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression Sajiki, Yamato Konnai, Satoru Ikenaka, Yoshinori Gulay, Kevin Christian Montecillo Kobayashi, Atsushi Parizi, Luís Fernando João, Benvindo Capela Watari, Kei Fujisawa, Sotaro Okagawa, Tomohiro Maekawa, Naoya Logullo, Carlos da Silva Vaz, Itabajara Murata, Shiro Ohashi, Kazuhiko Sci Rep Article The tick Rhipicephalus microplus is a harmful parasite of cattle that causes considerable economic losses to the cattle breeding industry. Although R. microplus saliva (Rm-saliva) contains several immunosuppressants, any association between Rm-saliva and the expression of immunoinhibitory molecules, such as programmed death (PD)-1 and PD-ligand 1 (PD-L1), has not been described. In this study, flow cytometric analyses revealed that Rm-saliva upregulated PD-1 expression in T cells and PD-L1 expression in CD14(+) and CD11c(+) cells in cattle. Additionally, Rm-saliva decreased CD69 expression in T cells and Th1 cytokine production from peripheral blood mononuclear cells. Furthermore, PD-L1 blockade increased IFN-γ production in the presence of Rm-saliva, suggesting that Rm-saliva suppresses Th1 responses via the PD-1/PD-L1 pathway. To reveal the upregulation mechanism of PD-1/PD-L1 by Rm-saliva, we analyzed the function of prostaglandin E(2) (PGE(2)), which is known as an inducer of PD-L1 expression, in Rm-saliva. We found that Rm-saliva contained a high concentration of PGE(2), and PGE(2) treatment induced PD-L1 expression in CD14(+) cells in vitro. Immunohistochemical analyses revealed that PGE(2) and PD-L1 expression was upregulated in tick-attached skin in cattle. These data suggest that PGE(2) in Rm-saliva has the potential to induce the expression of immunoinhibitory molecules in host immune cells. Nature Publishing Group UK 2021-01-13 /pmc/articles/PMC7806669/ /pubmed/33441793 http://dx.doi.org/10.1038/s41598-020-80251-y Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sajiki, Yamato
Konnai, Satoru
Ikenaka, Yoshinori
Gulay, Kevin Christian Montecillo
Kobayashi, Atsushi
Parizi, Luís Fernando
João, Benvindo Capela
Watari, Kei
Fujisawa, Sotaro
Okagawa, Tomohiro
Maekawa, Naoya
Logullo, Carlos
da Silva Vaz, Itabajara
Murata, Shiro
Ohashi, Kazuhiko
Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
title Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
title_full Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
title_fullStr Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
title_full_unstemmed Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
title_short Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
title_sort tick saliva-induced programmed death-1 and pd-ligand 1 and its related host immunosuppression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806669/
https://www.ncbi.nlm.nih.gov/pubmed/33441793
http://dx.doi.org/10.1038/s41598-020-80251-y
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