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Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
Microbial dysbiosis has been implicated in the pathogenesis of oral cancer. We analyzed the compositional and metabolic profile of the bacteriome in three specific niches in oral cancer patients along with controls using 16SrRNA sequencing (Illumina Miseq) and DADA2 software. We found major differen...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806708/ https://www.ncbi.nlm.nih.gov/pubmed/33441939 http://dx.doi.org/10.1038/s41598-020-80859-0 |
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author | Gopinath, Divya Menon, Rohit Kunnath Wie, Chong Chun Banerjee, Moinak Panda, Swagatika Mandal, Deviprasad Behera, Paresh Kumar Roychoudhury, Susanta Kheur, Supriya Botelho, Michael George Johnson, Newell W. |
author_facet | Gopinath, Divya Menon, Rohit Kunnath Wie, Chong Chun Banerjee, Moinak Panda, Swagatika Mandal, Deviprasad Behera, Paresh Kumar Roychoudhury, Susanta Kheur, Supriya Botelho, Michael George Johnson, Newell W. |
author_sort | Gopinath, Divya |
collection | PubMed |
description | Microbial dysbiosis has been implicated in the pathogenesis of oral cancer. We analyzed the compositional and metabolic profile of the bacteriome in three specific niches in oral cancer patients along with controls using 16SrRNA sequencing (Illumina Miseq) and DADA2 software. We found major differences between patients and control subjects. Bacterial communities associated with the tumor surface and deep paired tumor tissue differed significantly. Tumor surfaces carried elevated abundances of taxa belonging to genera Porphyromonas, Enterobacteriae, Neisseria, Streptococcus and Fusobacteria, whereas Prevotella, Treponema, Sphingomonas, Meiothermus and Mycoplasma genera were significantly more abundant in deep tissue. The most abundant microbial metabolic pathways were those related to fatty-acid biosynthesis, carbon metabolism and amino-acid metabolism on the tumor surface: carbohydrate metabolism and organic polymer degradation were elevated in tumor tissues. The bacteriome of saliva from patients with oral cancer differed significantly from paired tumor tissue in terms of community structure, however remained similar at taxonomic and metabolic levels except for elevated abundances of Streptococcus, Lactobacillus and Bacteroides, and acetoin-biosynthesis, respectively. These shifts to a pro-inflammatory profile are consistent with other studies suggesting oncogenic properties. Importantly, selection of the principal source of microbial DNA is key to ensure reliable, reproducible and comparable results in microbiome studies. |
format | Online Article Text |
id | pubmed-7806708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78067082021-01-14 Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer Gopinath, Divya Menon, Rohit Kunnath Wie, Chong Chun Banerjee, Moinak Panda, Swagatika Mandal, Deviprasad Behera, Paresh Kumar Roychoudhury, Susanta Kheur, Supriya Botelho, Michael George Johnson, Newell W. Sci Rep Article Microbial dysbiosis has been implicated in the pathogenesis of oral cancer. We analyzed the compositional and metabolic profile of the bacteriome in three specific niches in oral cancer patients along with controls using 16SrRNA sequencing (Illumina Miseq) and DADA2 software. We found major differences between patients and control subjects. Bacterial communities associated with the tumor surface and deep paired tumor tissue differed significantly. Tumor surfaces carried elevated abundances of taxa belonging to genera Porphyromonas, Enterobacteriae, Neisseria, Streptococcus and Fusobacteria, whereas Prevotella, Treponema, Sphingomonas, Meiothermus and Mycoplasma genera were significantly more abundant in deep tissue. The most abundant microbial metabolic pathways were those related to fatty-acid biosynthesis, carbon metabolism and amino-acid metabolism on the tumor surface: carbohydrate metabolism and organic polymer degradation were elevated in tumor tissues. The bacteriome of saliva from patients with oral cancer differed significantly from paired tumor tissue in terms of community structure, however remained similar at taxonomic and metabolic levels except for elevated abundances of Streptococcus, Lactobacillus and Bacteroides, and acetoin-biosynthesis, respectively. These shifts to a pro-inflammatory profile are consistent with other studies suggesting oncogenic properties. Importantly, selection of the principal source of microbial DNA is key to ensure reliable, reproducible and comparable results in microbiome studies. Nature Publishing Group UK 2021-01-13 /pmc/articles/PMC7806708/ /pubmed/33441939 http://dx.doi.org/10.1038/s41598-020-80859-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gopinath, Divya Menon, Rohit Kunnath Wie, Chong Chun Banerjee, Moinak Panda, Swagatika Mandal, Deviprasad Behera, Paresh Kumar Roychoudhury, Susanta Kheur, Supriya Botelho, Michael George Johnson, Newell W. Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer |
title | Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer |
title_full | Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer |
title_fullStr | Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer |
title_full_unstemmed | Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer |
title_short | Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer |
title_sort | differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806708/ https://www.ncbi.nlm.nih.gov/pubmed/33441939 http://dx.doi.org/10.1038/s41598-020-80859-0 |
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