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The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization
Candida glabrata is an opportunistic human fungal pathogen and is frequently present in the human microbiome. It has a high relative resistance to environmental stresses and several antifungal drugs. An important component involved in microbial stress tolerance is trehalose. In this work, we charact...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808424/ https://www.ncbi.nlm.nih.gov/pubmed/33356857 http://dx.doi.org/10.1080/21505594.2020.1868825 |
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author | Van Ende, Mieke Timmermans, Bea Vanreppelen, Giel Siscar-Lewin, Sofía Fischer, Daniel Wijnants, Stefanie Romero, Celia Lobo Yazdani, Saleh Rogiers, Ona Demuyser, Liesbeth Van Zeebroeck, Griet Cen, Yuke Kuchler, Karl Brunke, Sascha Van Dijck, Patrick |
author_facet | Van Ende, Mieke Timmermans, Bea Vanreppelen, Giel Siscar-Lewin, Sofía Fischer, Daniel Wijnants, Stefanie Romero, Celia Lobo Yazdani, Saleh Rogiers, Ona Demuyser, Liesbeth Van Zeebroeck, Griet Cen, Yuke Kuchler, Karl Brunke, Sascha Van Dijck, Patrick |
author_sort | Van Ende, Mieke |
collection | PubMed |
description | Candida glabrata is an opportunistic human fungal pathogen and is frequently present in the human microbiome. It has a high relative resistance to environmental stresses and several antifungal drugs. An important component involved in microbial stress tolerance is trehalose. In this work, we characterized the three C. glabrata trehalase enzymes Ath1, Nth1 and Nth2. Single, double and triple deletion strains were constructed and characterized both in vitro and in vivo to determine the role of these enzymes in virulence. Ath1 was found to be located in the periplasm and was essential for growth on trehalose as sole carbon source, while Nth1 on the other hand was important for oxidative stress resistance, an observation which was consistent by the lower survival rate of the NTH1 deletion strain in human macrophages. No significant phenotype was observed for Nth2. The triple deletion strain was unable to establish a stable colonization of the gastrointestinal (GI) tract in mice indicating the importance of having trehalase activity for colonization in the gut. |
format | Online Article Text |
id | pubmed-7808424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78084242021-01-29 The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization Van Ende, Mieke Timmermans, Bea Vanreppelen, Giel Siscar-Lewin, Sofía Fischer, Daniel Wijnants, Stefanie Romero, Celia Lobo Yazdani, Saleh Rogiers, Ona Demuyser, Liesbeth Van Zeebroeck, Griet Cen, Yuke Kuchler, Karl Brunke, Sascha Van Dijck, Patrick Virulence Research Paper Candida glabrata is an opportunistic human fungal pathogen and is frequently present in the human microbiome. It has a high relative resistance to environmental stresses and several antifungal drugs. An important component involved in microbial stress tolerance is trehalose. In this work, we characterized the three C. glabrata trehalase enzymes Ath1, Nth1 and Nth2. Single, double and triple deletion strains were constructed and characterized both in vitro and in vivo to determine the role of these enzymes in virulence. Ath1 was found to be located in the periplasm and was essential for growth on trehalose as sole carbon source, while Nth1 on the other hand was important for oxidative stress resistance, an observation which was consistent by the lower survival rate of the NTH1 deletion strain in human macrophages. No significant phenotype was observed for Nth2. The triple deletion strain was unable to establish a stable colonization of the gastrointestinal (GI) tract in mice indicating the importance of having trehalase activity for colonization in the gut. Taylor & Francis 2020-12-28 /pmc/articles/PMC7808424/ /pubmed/33356857 http://dx.doi.org/10.1080/21505594.2020.1868825 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Van Ende, Mieke Timmermans, Bea Vanreppelen, Giel Siscar-Lewin, Sofía Fischer, Daniel Wijnants, Stefanie Romero, Celia Lobo Yazdani, Saleh Rogiers, Ona Demuyser, Liesbeth Van Zeebroeck, Griet Cen, Yuke Kuchler, Karl Brunke, Sascha Van Dijck, Patrick The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization |
title | The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization |
title_full | The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization |
title_fullStr | The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization |
title_full_unstemmed | The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization |
title_short | The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization |
title_sort | involvement of the candida glabrata trehalase enzymes in stress resistance and gut colonization |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808424/ https://www.ncbi.nlm.nih.gov/pubmed/33356857 http://dx.doi.org/10.1080/21505594.2020.1868825 |
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