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Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice
Intracranial self-stimulation, in which an animal performs an operant response to receive regional brain electrical stimulation, is a widely used procedure to study motivated behavior. While local neuronal activity has long been measured immediately before or after the operant, imaging the whole bra...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809041/ https://www.ncbi.nlm.nih.gov/pubmed/33446857 http://dx.doi.org/10.1038/s42003-020-01612-x |
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author | Cover, Christopher G. Kesner, Andrew J. Ukani, Shehzad Stein, Elliot A. Ikemoto, Satoshi Yang, Yihong Lu, Hanbing |
author_facet | Cover, Christopher G. Kesner, Andrew J. Ukani, Shehzad Stein, Elliot A. Ikemoto, Satoshi Yang, Yihong Lu, Hanbing |
author_sort | Cover, Christopher G. |
collection | PubMed |
description | Intracranial self-stimulation, in which an animal performs an operant response to receive regional brain electrical stimulation, is a widely used procedure to study motivated behavior. While local neuronal activity has long been measured immediately before or after the operant, imaging the whole brain in real-time remains a challenge. Herein we report a method that permits functional MRI (fMRI) of brain dynamics while mice are cued to perform an operant task: licking a spout to receive optogenetic stimulation to the medial prefrontal cortex (MPFC) during a cue ON, but not cue OFF. Licking during cue ON results in activation of a widely distributed network consistent with underlying MPFC projections, while licking during cue OFF (without optogenetic stimulation) leads to negative fMRI signal in brain regions involved in acute extinction. Noninvasive whole brain readout combined with circuit-specific neuromodulation opens an avenue for investigating adaptive behavior in both healthy and disease models. |
format | Online Article Text |
id | pubmed-7809041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78090412021-01-21 Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice Cover, Christopher G. Kesner, Andrew J. Ukani, Shehzad Stein, Elliot A. Ikemoto, Satoshi Yang, Yihong Lu, Hanbing Commun Biol Article Intracranial self-stimulation, in which an animal performs an operant response to receive regional brain electrical stimulation, is a widely used procedure to study motivated behavior. While local neuronal activity has long been measured immediately before or after the operant, imaging the whole brain in real-time remains a challenge. Herein we report a method that permits functional MRI (fMRI) of brain dynamics while mice are cued to perform an operant task: licking a spout to receive optogenetic stimulation to the medial prefrontal cortex (MPFC) during a cue ON, but not cue OFF. Licking during cue ON results in activation of a widely distributed network consistent with underlying MPFC projections, while licking during cue OFF (without optogenetic stimulation) leads to negative fMRI signal in brain regions involved in acute extinction. Noninvasive whole brain readout combined with circuit-specific neuromodulation opens an avenue for investigating adaptive behavior in both healthy and disease models. Nature Publishing Group UK 2021-01-14 /pmc/articles/PMC7809041/ /pubmed/33446857 http://dx.doi.org/10.1038/s42003-020-01612-x Text en © This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Cover, Christopher G. Kesner, Andrew J. Ukani, Shehzad Stein, Elliot A. Ikemoto, Satoshi Yang, Yihong Lu, Hanbing Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice |
title | Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice |
title_full | Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice |
title_fullStr | Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice |
title_full_unstemmed | Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice |
title_short | Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice |
title_sort | whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809041/ https://www.ncbi.nlm.nih.gov/pubmed/33446857 http://dx.doi.org/10.1038/s42003-020-01612-x |
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