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The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria

BACKGROUND: Avr-Pita was the first effector identified in the blast fungus (Magnaporthe oryzae)–rice (Oryza sativa) pathosystem. However, the molecular mechanism underlying its effects on the host plant has remained a long-standing mystery. RESULTS: Here, we report that ectopically expressing Avr-Pi...

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Autores principales: Han, Jingluan, Wang, Xiaoyu, Wang, Fengpin, Zhao, Zhe, Li, Gousi, Zhu, Xiaoyuan, Su, Jing, Chen, Letian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809080/
https://www.ncbi.nlm.nih.gov/pubmed/33443630
http://dx.doi.org/10.1186/s12284-021-00453-4
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author Han, Jingluan
Wang, Xiaoyu
Wang, Fengpin
Zhao, Zhe
Li, Gousi
Zhu, Xiaoyuan
Su, Jing
Chen, Letian
author_facet Han, Jingluan
Wang, Xiaoyu
Wang, Fengpin
Zhao, Zhe
Li, Gousi
Zhu, Xiaoyuan
Su, Jing
Chen, Letian
author_sort Han, Jingluan
collection PubMed
description BACKGROUND: Avr-Pita was the first effector identified in the blast fungus (Magnaporthe oryzae)–rice (Oryza sativa) pathosystem. However, the molecular mechanism underlying its effects on the host plant has remained a long-standing mystery. RESULTS: Here, we report that ectopically expressing Avr-Pita in rice enhances susceptibility to M. oryzae and suppresses pathogen-associated molecular pattern (PAMP)-triggered defense responses. Avr-Pita targets the host mitochondria and interacts with the cytochrome c oxidase (COX) assembly protein OsCOX11, a key regulator of mitochondrial reactive oxygen species (ROS) metabolism in rice. Overexpressing Avr-Pita or OsCOX11 increased COX activity and decreased ROS accumulation triggered by the fungal PAMP chitin. OsCOX11-overexpressing plants showed increased susceptibility to M. oryzae, whereas OsCOX11-knockdown plants showed resistance to M. oryzae. CONCLUSIONS: Taken together, these findings suggest that the fungal pathogen M. oryzae delivers the effector Avr-Pita to the host plant, where it enhances COX activity thus decreasing ROS accumulation. Therefore, this effector suppresses host innate immunity by perturbing ROS metabolism in the mitochondria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-021-00453-4.
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spelling pubmed-78090802021-01-21 The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria Han, Jingluan Wang, Xiaoyu Wang, Fengpin Zhao, Zhe Li, Gousi Zhu, Xiaoyuan Su, Jing Chen, Letian Rice (N Y) Original Article BACKGROUND: Avr-Pita was the first effector identified in the blast fungus (Magnaporthe oryzae)–rice (Oryza sativa) pathosystem. However, the molecular mechanism underlying its effects on the host plant has remained a long-standing mystery. RESULTS: Here, we report that ectopically expressing Avr-Pita in rice enhances susceptibility to M. oryzae and suppresses pathogen-associated molecular pattern (PAMP)-triggered defense responses. Avr-Pita targets the host mitochondria and interacts with the cytochrome c oxidase (COX) assembly protein OsCOX11, a key regulator of mitochondrial reactive oxygen species (ROS) metabolism in rice. Overexpressing Avr-Pita or OsCOX11 increased COX activity and decreased ROS accumulation triggered by the fungal PAMP chitin. OsCOX11-overexpressing plants showed increased susceptibility to M. oryzae, whereas OsCOX11-knockdown plants showed resistance to M. oryzae. CONCLUSIONS: Taken together, these findings suggest that the fungal pathogen M. oryzae delivers the effector Avr-Pita to the host plant, where it enhances COX activity thus decreasing ROS accumulation. Therefore, this effector suppresses host innate immunity by perturbing ROS metabolism in the mitochondria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-021-00453-4. Springer US 2021-01-14 /pmc/articles/PMC7809080/ /pubmed/33443630 http://dx.doi.org/10.1186/s12284-021-00453-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Han, Jingluan
Wang, Xiaoyu
Wang, Fengpin
Zhao, Zhe
Li, Gousi
Zhu, Xiaoyuan
Su, Jing
Chen, Letian
The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria
title The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria
title_full The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria
title_fullStr The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria
title_full_unstemmed The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria
title_short The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria
title_sort fungal effector avr-pita suppresses innate immunity by increasing cox activity in rice mitochondria
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809080/
https://www.ncbi.nlm.nih.gov/pubmed/33443630
http://dx.doi.org/10.1186/s12284-021-00453-4
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