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Cortisol AuPd plasmonic unclad POF biosensor
This paper presents the development and feasibility tests of a cortisol immunosensor. The sensor is based on surface plasmon resonance (SPR) using an unclad plastic optical fiber (POF) in which the SPR is used as sensitivity enhancer, promoted by a gold/palladium (AuPd) alloy coating. The AuPd coate...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809164/ https://www.ncbi.nlm.nih.gov/pubmed/33489788 http://dx.doi.org/10.1016/j.btre.2021.e00587 |
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author | Leitão, Cátia Leal-Junior, Arnaldo Almeida, Ana R. Pereira, Sónia O. Costa, Florinda M. Pinto, João L. Marques, C. |
author_facet | Leitão, Cátia Leal-Junior, Arnaldo Almeida, Ana R. Pereira, Sónia O. Costa, Florinda M. Pinto, João L. Marques, C. |
author_sort | Leitão, Cátia |
collection | PubMed |
description | This paper presents the development and feasibility tests of a cortisol immunosensor. The sensor is based on surface plasmon resonance (SPR) using an unclad plastic optical fiber (POF) in which the SPR is used as sensitivity enhancer, promoted by a gold/palladium (AuPd) alloy coating. The AuPd coated fibers were functionalized with an anti-cortisol antibody and passivated with bovine serum albumin (BSA) to be tested in the presence of cortisol as target analyte. The antibody-antigen binding reaction caused a variation of the refractive index on the surface of the AuPd coating, which leads to a shift of the SPR signature wavelength. The sensor was tested for different cortisol concentrations, ranging from 0.005 to 10 ng/mL. The reported biosensor presented a total wavelength shift of 15 nm for the testing range, putting in evidence a high sensitivity. Control tests for selectivity assessment were also performed. Concentrations as high as 10 ng/mL of cortisol, in a sensor functionalized with anti-hCG antibodies, only resulted in 1 nm variation of the resonance wavelength, 15 times lower than the one functionalized with the anti-cortisol antibodies, which indicates a high selectivity for the proposed approach. For this sensing approach the limit of detection (LOD) was determined to be 1 pg/mL. The proposed SPR based POF sensor has a low-cost interrogation method, high sensitivity and low LOD, straightforward signal processing and find important applications in different biological fields. |
format | Online Article Text |
id | pubmed-7809164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-78091642021-01-22 Cortisol AuPd plasmonic unclad POF biosensor Leitão, Cátia Leal-Junior, Arnaldo Almeida, Ana R. Pereira, Sónia O. Costa, Florinda M. Pinto, João L. Marques, C. Biotechnol Rep (Amst) Research Article This paper presents the development and feasibility tests of a cortisol immunosensor. The sensor is based on surface plasmon resonance (SPR) using an unclad plastic optical fiber (POF) in which the SPR is used as sensitivity enhancer, promoted by a gold/palladium (AuPd) alloy coating. The AuPd coated fibers were functionalized with an anti-cortisol antibody and passivated with bovine serum albumin (BSA) to be tested in the presence of cortisol as target analyte. The antibody-antigen binding reaction caused a variation of the refractive index on the surface of the AuPd coating, which leads to a shift of the SPR signature wavelength. The sensor was tested for different cortisol concentrations, ranging from 0.005 to 10 ng/mL. The reported biosensor presented a total wavelength shift of 15 nm for the testing range, putting in evidence a high sensitivity. Control tests for selectivity assessment were also performed. Concentrations as high as 10 ng/mL of cortisol, in a sensor functionalized with anti-hCG antibodies, only resulted in 1 nm variation of the resonance wavelength, 15 times lower than the one functionalized with the anti-cortisol antibodies, which indicates a high selectivity for the proposed approach. For this sensing approach the limit of detection (LOD) was determined to be 1 pg/mL. The proposed SPR based POF sensor has a low-cost interrogation method, high sensitivity and low LOD, straightforward signal processing and find important applications in different biological fields. Elsevier 2021-01-06 /pmc/articles/PMC7809164/ /pubmed/33489788 http://dx.doi.org/10.1016/j.btre.2021.e00587 Text en © 2021 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Leitão, Cátia Leal-Junior, Arnaldo Almeida, Ana R. Pereira, Sónia O. Costa, Florinda M. Pinto, João L. Marques, C. Cortisol AuPd plasmonic unclad POF biosensor |
title | Cortisol AuPd plasmonic unclad POF biosensor |
title_full | Cortisol AuPd plasmonic unclad POF biosensor |
title_fullStr | Cortisol AuPd plasmonic unclad POF biosensor |
title_full_unstemmed | Cortisol AuPd plasmonic unclad POF biosensor |
title_short | Cortisol AuPd plasmonic unclad POF biosensor |
title_sort | cortisol aupd plasmonic unclad pof biosensor |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809164/ https://www.ncbi.nlm.nih.gov/pubmed/33489788 http://dx.doi.org/10.1016/j.btre.2021.e00587 |
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