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S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells

To understand the potential effects of cancer cells on surrounding normal mammary epithelial cells, we performed direct co-culture of non-tumorigenic mammary epithelial MCF10A cells and various breast cancer cells. Firstly, we observed dynamic cell–cell interactions between the MCF10A cells and brea...

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Autores principales: Jo, Seol Hwa, Heo, Woo Hang, Son, Hye-Youn, Quan, Mingji, Hong, Bok Sil, Kim, Ju Hee, Lee, Han-Byoel, Han, Wonshik, Park, Yeonju, Lee, Dong-Sup, Kwon, Nam Hoon, Park, Min Chul, Chae, Jeesoo, Kim, Jong-Il, Noh, Dong-Young, Moon, Hyeong-Gon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809201/
https://www.ncbi.nlm.nih.gov/pubmed/33446797
http://dx.doi.org/10.1038/s41598-020-80625-2
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author Jo, Seol Hwa
Heo, Woo Hang
Son, Hye-Youn
Quan, Mingji
Hong, Bok Sil
Kim, Ju Hee
Lee, Han-Byoel
Han, Wonshik
Park, Yeonju
Lee, Dong-Sup
Kwon, Nam Hoon
Park, Min Chul
Chae, Jeesoo
Kim, Jong-Il
Noh, Dong-Young
Moon, Hyeong-Gon
author_facet Jo, Seol Hwa
Heo, Woo Hang
Son, Hye-Youn
Quan, Mingji
Hong, Bok Sil
Kim, Ju Hee
Lee, Han-Byoel
Han, Wonshik
Park, Yeonju
Lee, Dong-Sup
Kwon, Nam Hoon
Park, Min Chul
Chae, Jeesoo
Kim, Jong-Il
Noh, Dong-Young
Moon, Hyeong-Gon
author_sort Jo, Seol Hwa
collection PubMed
description To understand the potential effects of cancer cells on surrounding normal mammary epithelial cells, we performed direct co-culture of non-tumorigenic mammary epithelial MCF10A cells and various breast cancer cells. Firstly, we observed dynamic cell–cell interactions between the MCF10A cells and breast cancer cells including lamellipodia or nanotube-like contacts and transfer of extracellular vesicles. Co-cultured MCF10A cells exhibited features of epithelial-mesenchymal transition, and showed increased capacity of cell proliferation, migration, colony formation, and 3-dimensional sphere formation. Direct co-culture showed most distinct phenotype changes in MCF10A cells followed by conditioned media treatment and indirect co-culture. Transcriptome analysis and phosphor-protein array suggested that several cancer-related pathways are significantly dysregulated in MCF10A cells after the direct co-culture with breast cancer cells. S100A8 and S100A9 showed distinct up-regulation in the co-cultured MCF10A cells and their microenvironmental upregulation was also observed in the orthotropic xenograft of syngeneic mouse mammary tumors. When S100A8/A9 overexpression was induced in MCF10A cells, the cells showed phenotypic features of directly co-cultured MCF10A cells in terms of in vitro cell behaviors and signaling activities suggesting a S100A8/A9-mediated transition program in non-tumorigenic epithelial cells. This study suggests the possibility of dynamic cell–cell interactions between non-tumorigenic mammary epithelial cells and breast cancer cells that could lead to a substantial transition in molecular and functional characteristics of mammary epithelial cells.
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spelling pubmed-78092012021-01-15 S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells Jo, Seol Hwa Heo, Woo Hang Son, Hye-Youn Quan, Mingji Hong, Bok Sil Kim, Ju Hee Lee, Han-Byoel Han, Wonshik Park, Yeonju Lee, Dong-Sup Kwon, Nam Hoon Park, Min Chul Chae, Jeesoo Kim, Jong-Il Noh, Dong-Young Moon, Hyeong-Gon Sci Rep Article To understand the potential effects of cancer cells on surrounding normal mammary epithelial cells, we performed direct co-culture of non-tumorigenic mammary epithelial MCF10A cells and various breast cancer cells. Firstly, we observed dynamic cell–cell interactions between the MCF10A cells and breast cancer cells including lamellipodia or nanotube-like contacts and transfer of extracellular vesicles. Co-cultured MCF10A cells exhibited features of epithelial-mesenchymal transition, and showed increased capacity of cell proliferation, migration, colony formation, and 3-dimensional sphere formation. Direct co-culture showed most distinct phenotype changes in MCF10A cells followed by conditioned media treatment and indirect co-culture. Transcriptome analysis and phosphor-protein array suggested that several cancer-related pathways are significantly dysregulated in MCF10A cells after the direct co-culture with breast cancer cells. S100A8 and S100A9 showed distinct up-regulation in the co-cultured MCF10A cells and their microenvironmental upregulation was also observed in the orthotropic xenograft of syngeneic mouse mammary tumors. When S100A8/A9 overexpression was induced in MCF10A cells, the cells showed phenotypic features of directly co-cultured MCF10A cells in terms of in vitro cell behaviors and signaling activities suggesting a S100A8/A9-mediated transition program in non-tumorigenic epithelial cells. This study suggests the possibility of dynamic cell–cell interactions between non-tumorigenic mammary epithelial cells and breast cancer cells that could lead to a substantial transition in molecular and functional characteristics of mammary epithelial cells. Nature Publishing Group UK 2021-01-14 /pmc/articles/PMC7809201/ /pubmed/33446797 http://dx.doi.org/10.1038/s41598-020-80625-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jo, Seol Hwa
Heo, Woo Hang
Son, Hye-Youn
Quan, Mingji
Hong, Bok Sil
Kim, Ju Hee
Lee, Han-Byoel
Han, Wonshik
Park, Yeonju
Lee, Dong-Sup
Kwon, Nam Hoon
Park, Min Chul
Chae, Jeesoo
Kim, Jong-Il
Noh, Dong-Young
Moon, Hyeong-Gon
S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells
title S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells
title_full S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells
title_fullStr S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells
title_full_unstemmed S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells
title_short S100A8/A9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells
title_sort s100a8/a9 mediate the reprograming of normal mammary epithelial cells induced by dynamic cell–cell interactions with adjacent breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809201/
https://www.ncbi.nlm.nih.gov/pubmed/33446797
http://dx.doi.org/10.1038/s41598-020-80625-2
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