Protocol for isolation of spermatids from mouse testes

Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit...

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Detalles Bibliográficos
Autores principales: Kim, Chang Rok, Noda, Taichi, Okada, Yuki, Ikawa, Masahito, Baek, Sung Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809437/
https://www.ncbi.nlm.nih.gov/pubmed/33490974
http://dx.doi.org/10.1016/j.xpro.2020.100254
Descripción
Sumario:Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

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