Protocol for isolation of spermatids from mouse testes

Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit...

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Detalles Bibliográficos
Autores principales: Kim, Chang Rok, Noda, Taichi, Okada, Yuki, Ikawa, Masahito, Baek, Sung Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809437/
https://www.ncbi.nlm.nih.gov/pubmed/33490974
http://dx.doi.org/10.1016/j.xpro.2020.100254
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author Kim, Chang Rok
Noda, Taichi
Okada, Yuki
Ikawa, Masahito
Baek, Sung Hee
author_facet Kim, Chang Rok
Noda, Taichi
Okada, Yuki
Ikawa, Masahito
Baek, Sung Hee
author_sort Kim, Chang Rok
collection PubMed
description Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).
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spelling pubmed-78094372021-01-22 Protocol for isolation of spermatids from mouse testes Kim, Chang Rok Noda, Taichi Okada, Yuki Ikawa, Masahito Baek, Sung Hee STAR Protoc Protocol Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020). Elsevier 2021-01-08 /pmc/articles/PMC7809437/ /pubmed/33490974 http://dx.doi.org/10.1016/j.xpro.2020.100254 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kim, Chang Rok
Noda, Taichi
Okada, Yuki
Ikawa, Masahito
Baek, Sung Hee
Protocol for isolation of spermatids from mouse testes
title Protocol for isolation of spermatids from mouse testes
title_full Protocol for isolation of spermatids from mouse testes
title_fullStr Protocol for isolation of spermatids from mouse testes
title_full_unstemmed Protocol for isolation of spermatids from mouse testes
title_short Protocol for isolation of spermatids from mouse testes
title_sort protocol for isolation of spermatids from mouse testes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809437/
https://www.ncbi.nlm.nih.gov/pubmed/33490974
http://dx.doi.org/10.1016/j.xpro.2020.100254
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