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New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures

[Image: see text] Photoemission electron microscopy and imaging X-ray photoelectron spectroscopy are today frequently used to obtain chemical and electronic states, chemical shifts, work function profiles within the fields of surface- and material sciences. Lately, because of recent technological ad...

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Autores principales: Skallberg, Andreas, Bunnfors, Kalle, Brommesson, Caroline, Uvdal, Kajsa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809688/
https://www.ncbi.nlm.nih.gov/pubmed/33263404
http://dx.doi.org/10.1021/acs.nanolett.0c03554
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author Skallberg, Andreas
Bunnfors, Kalle
Brommesson, Caroline
Uvdal, Kajsa
author_facet Skallberg, Andreas
Bunnfors, Kalle
Brommesson, Caroline
Uvdal, Kajsa
author_sort Skallberg, Andreas
collection PubMed
description [Image: see text] Photoemission electron microscopy and imaging X-ray photoelectron spectroscopy are today frequently used to obtain chemical and electronic states, chemical shifts, work function profiles within the fields of surface- and material sciences. Lately, because of recent technological advances, these tools have also been valuable within life sciences. In this study, we have investigated the power of photoemission electron microscopy and imaging X-ray photoelectron spectroscopy for visualization of human neutrophil granulocytes. These cells, commonly called neutrophils, are essential for our innate immune system. We hereby investigate the structure and morphology of neutrophils when adhered to gold and silicon surfaces. Energy-filtered imaging of single cells are acquired. The characteristic polymorphonuclear cellular nuclei divided into 2–5 lobes is visualized. Element-specific imaging is achieved based on O 1s, P 2p, C 1s, Si 2p, and N 1s core level spectra, delivering elemental distribution with submicrometer resolution, illustrating the strength of this type of cellular morphological studies.
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spelling pubmed-78096882021-01-15 New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures Skallberg, Andreas Bunnfors, Kalle Brommesson, Caroline Uvdal, Kajsa Nano Lett [Image: see text] Photoemission electron microscopy and imaging X-ray photoelectron spectroscopy are today frequently used to obtain chemical and electronic states, chemical shifts, work function profiles within the fields of surface- and material sciences. Lately, because of recent technological advances, these tools have also been valuable within life sciences. In this study, we have investigated the power of photoemission electron microscopy and imaging X-ray photoelectron spectroscopy for visualization of human neutrophil granulocytes. These cells, commonly called neutrophils, are essential for our innate immune system. We hereby investigate the structure and morphology of neutrophils when adhered to gold and silicon surfaces. Energy-filtered imaging of single cells are acquired. The characteristic polymorphonuclear cellular nuclei divided into 2–5 lobes is visualized. Element-specific imaging is achieved based on O 1s, P 2p, C 1s, Si 2p, and N 1s core level spectra, delivering elemental distribution with submicrometer resolution, illustrating the strength of this type of cellular morphological studies. American Chemical Society 2020-12-02 2021-01-13 /pmc/articles/PMC7809688/ /pubmed/33263404 http://dx.doi.org/10.1021/acs.nanolett.0c03554 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Skallberg, Andreas
Bunnfors, Kalle
Brommesson, Caroline
Uvdal, Kajsa
New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures
title New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures
title_full New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures
title_fullStr New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures
title_full_unstemmed New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures
title_short New Tools for Imaging Neutrophils: Work Function Mapping and Element-Specific, Label-Free Imaging of Cellular Structures
title_sort new tools for imaging neutrophils: work function mapping and element-specific, label-free imaging of cellular structures
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809688/
https://www.ncbi.nlm.nih.gov/pubmed/33263404
http://dx.doi.org/10.1021/acs.nanolett.0c03554
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