Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals

Expanded human skin fibroblast cells from four different aged healthy individuals, 11-year-old female, 1-year-old male, 2-month-old male, and 8-year-old male, were used to generate integration-free induced pluripotent stem cell (iPSC) lines TRNDi021-C, TRNDi023-D, TRNDi024-D, and TRNDi025-A, respect...

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Autores principales: Xu, Xiaogang, Pradhan, Manisha, Xu, Miao, Cheng, Yu-Shan, Beers, Jeanette, Linask, Kaari L., Lin, Yongshun, Zheng, Wei, Zou, Jizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810501/
https://www.ncbi.nlm.nih.gov/pubmed/33038742
http://dx.doi.org/10.1016/j.scr.2020.102011
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author Xu, Xiaogang
Pradhan, Manisha
Xu, Miao
Cheng, Yu-Shan
Beers, Jeanette
Linask, Kaari L.
Lin, Yongshun
Zheng, Wei
Zou, Jizhong
author_facet Xu, Xiaogang
Pradhan, Manisha
Xu, Miao
Cheng, Yu-Shan
Beers, Jeanette
Linask, Kaari L.
Lin, Yongshun
Zheng, Wei
Zou, Jizhong
author_sort Xu, Xiaogang
collection PubMed
description Expanded human skin fibroblast cells from four different aged healthy individuals, 11-year-old female, 1-year-old male, 2-month-old male, and 8-year-old male, were used to generate integration-free induced pluripotent stem cell (iPSC) lines TRNDi021-C, TRNDi023-D, TRNDi024-D, and TRNDi025-A, respectively, by exogenous expression of four reprogramming factors, human SXO2, OCT3/4, C-MYC, KLF4. The authenticity of established iPSC lines was confirmed by the expressions of stem cell markers, karyotype analysis, and teratoma formation. These iPSC lines could serve as young healthy controls for the studies involving patient-specific iPSCs.
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spelling pubmed-78105012021-01-15 Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals Xu, Xiaogang Pradhan, Manisha Xu, Miao Cheng, Yu-Shan Beers, Jeanette Linask, Kaari L. Lin, Yongshun Zheng, Wei Zou, Jizhong Stem Cell Res Article Expanded human skin fibroblast cells from four different aged healthy individuals, 11-year-old female, 1-year-old male, 2-month-old male, and 8-year-old male, were used to generate integration-free induced pluripotent stem cell (iPSC) lines TRNDi021-C, TRNDi023-D, TRNDi024-D, and TRNDi025-A, respectively, by exogenous expression of four reprogramming factors, human SXO2, OCT3/4, C-MYC, KLF4. The authenticity of established iPSC lines was confirmed by the expressions of stem cell markers, karyotype analysis, and teratoma formation. These iPSC lines could serve as young healthy controls for the studies involving patient-specific iPSCs. 2020-10-01 2020-12 /pmc/articles/PMC7810501/ /pubmed/33038742 http://dx.doi.org/10.1016/j.scr.2020.102011 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Xu, Xiaogang
Pradhan, Manisha
Xu, Miao
Cheng, Yu-Shan
Beers, Jeanette
Linask, Kaari L.
Lin, Yongshun
Zheng, Wei
Zou, Jizhong
Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals
title Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals
title_full Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals
title_fullStr Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals
title_full_unstemmed Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals
title_short Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals
title_sort four induced pluripotent stem cell lines (trndi021-c, trndi023-d, trndi024-d and trndi025-a) generated from fibroblasts of four healthy individuals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810501/
https://www.ncbi.nlm.nih.gov/pubmed/33038742
http://dx.doi.org/10.1016/j.scr.2020.102011
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