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Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants

Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted of multiple, highly repetitive rDNA genes, encoding the catalytic ribosomal core RNAs which are transcribed from 45S rDNA units. Thei...

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Autores principales: Sims, Jason, Sestini, Giovanni, Elgert, Christiane, von Haeseler, Arndt, Schlögelhofer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810690/
https://www.ncbi.nlm.nih.gov/pubmed/33452254
http://dx.doi.org/10.1038/s41467-020-20728-6
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author Sims, Jason
Sestini, Giovanni
Elgert, Christiane
von Haeseler, Arndt
Schlögelhofer, Peter
author_facet Sims, Jason
Sestini, Giovanni
Elgert, Christiane
von Haeseler, Arndt
Schlögelhofer, Peter
author_sort Sims, Jason
collection PubMed
description Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted of multiple, highly repetitive rDNA genes, encoding the catalytic ribosomal core RNAs which are transcribed from 45S rDNA units. Their precise sequence information and organization remain uncharacterized. Here, using a combination of long- and short-read sequencing technologies we assemble contigs of the Arabidopsis NOR2 rDNA domain. We identify several expressed rRNA gene variants which are integrated into translating ribosomes in a tissue-specific manner. These findings support the concept of tissue specific ribosome subpopulations that differ in their rRNA composition and provide insights into the higher order organization of NOR2.
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spelling pubmed-78106902021-01-21 Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants Sims, Jason Sestini, Giovanni Elgert, Christiane von Haeseler, Arndt Schlögelhofer, Peter Nat Commun Article Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted of multiple, highly repetitive rDNA genes, encoding the catalytic ribosomal core RNAs which are transcribed from 45S rDNA units. Their precise sequence information and organization remain uncharacterized. Here, using a combination of long- and short-read sequencing technologies we assemble contigs of the Arabidopsis NOR2 rDNA domain. We identify several expressed rRNA gene variants which are integrated into translating ribosomes in a tissue-specific manner. These findings support the concept of tissue specific ribosome subpopulations that differ in their rRNA composition and provide insights into the higher order organization of NOR2. Nature Publishing Group UK 2021-01-15 /pmc/articles/PMC7810690/ /pubmed/33452254 http://dx.doi.org/10.1038/s41467-020-20728-6 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sims, Jason
Sestini, Giovanni
Elgert, Christiane
von Haeseler, Arndt
Schlögelhofer, Peter
Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants
title Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants
title_full Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants
title_fullStr Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants
title_full_unstemmed Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants
title_short Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants
title_sort sequencing of the arabidopsis nor2 reveals its distinct organization and tissue-specific rrna ribosomal variants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810690/
https://www.ncbi.nlm.nih.gov/pubmed/33452254
http://dx.doi.org/10.1038/s41467-020-20728-6
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