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Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
PURPOSE: Today, the development of wounds and their side effects has become a problematic issue in medical science research. Hydrogel-based dressings are some of the best candidates for this purpose due to their ability to keep the wound bed clean, as well as provide proper moisture, tissue compatib...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810733/ https://www.ncbi.nlm.nih.gov/pubmed/33469282 http://dx.doi.org/10.2147/IJN.S266692 |
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author | Ghasemian Lemraski, Ensieh Jahangirian, Hossein Dashti, Maryam Khajehali, Elaheh Sharafinia, Soheila Rafiee-Moghaddam, Roshanak Webster, Thomas J |
author_facet | Ghasemian Lemraski, Ensieh Jahangirian, Hossein Dashti, Maryam Khajehali, Elaheh Sharafinia, Soheila Rafiee-Moghaddam, Roshanak Webster, Thomas J |
author_sort | Ghasemian Lemraski, Ensieh |
collection | PubMed |
description | PURPOSE: Today, the development of wounds and their side effects has become a problematic issue in medical science research. Hydrogel-based dressings are some of the best candidates for this purpose due to their ability to keep the wound bed clean, as well as provide proper moisture, tissue compatibility and an antimicrobial effect for wound healing. On the other hand, copper and its compounds have been used experimentally for many years in studies as an antimicrobial substance. Various studies have been performed determining the antimicrobial properties of this element, during which significant effects on infection have been shown. METHODS: Chitosan/polyvinyl alcohol/copper nanofibers were successfully prepared by incorporating Cu onto a polymer electrospun using an electrospinning technique. A double-layer nanofiber composed of poly(vinyl alcohol) and chitosan incorporated with Cu nanoparticles as a protective layer and a second layer composed of polyvinylpyrrolidone (PVP) nanofibers which was adjacent to the damaged cells was prepared. The prepared nanofiber was characterized by TGA, FT-IR, TEM, SEM-EDS, and X-ray powder diffraction. The antimicrobial efficiency of the nanofibers was demonstrated through biological tests on some Gram-positive and Gram-negative bacteria. Finally, the prepared hydrogel formulations were prepared to evaluate their effect on the healing process of rat open wounds. RESULTS: In this study, data from SEM, TEM, EDS, and XRD confirmed the formation of uniform fibers with nanodiameters and the presence of Cu nanoparticles onto the electrospun nanofibers. The antibacterial activity of copper was observed against all of the selected bacteria, but the Gram-positive bacteria were more sensitive compared to Gram-negative bacteria. CONCLUSION: According to the obtained results, the hydrogel wound dressing prepared in this research can be effective in caring for open wounds in the early stages of wound healing and preventing the occurrence of prolonged open wounds. |
format | Online Article Text |
id | pubmed-7810733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-78107332021-01-18 Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment Ghasemian Lemraski, Ensieh Jahangirian, Hossein Dashti, Maryam Khajehali, Elaheh Sharafinia, Soheila Rafiee-Moghaddam, Roshanak Webster, Thomas J Int J Nanomedicine Original Research PURPOSE: Today, the development of wounds and their side effects has become a problematic issue in medical science research. Hydrogel-based dressings are some of the best candidates for this purpose due to their ability to keep the wound bed clean, as well as provide proper moisture, tissue compatibility and an antimicrobial effect for wound healing. On the other hand, copper and its compounds have been used experimentally for many years in studies as an antimicrobial substance. Various studies have been performed determining the antimicrobial properties of this element, during which significant effects on infection have been shown. METHODS: Chitosan/polyvinyl alcohol/copper nanofibers were successfully prepared by incorporating Cu onto a polymer electrospun using an electrospinning technique. A double-layer nanofiber composed of poly(vinyl alcohol) and chitosan incorporated with Cu nanoparticles as a protective layer and a second layer composed of polyvinylpyrrolidone (PVP) nanofibers which was adjacent to the damaged cells was prepared. The prepared nanofiber was characterized by TGA, FT-IR, TEM, SEM-EDS, and X-ray powder diffraction. The antimicrobial efficiency of the nanofibers was demonstrated through biological tests on some Gram-positive and Gram-negative bacteria. Finally, the prepared hydrogel formulations were prepared to evaluate their effect on the healing process of rat open wounds. RESULTS: In this study, data from SEM, TEM, EDS, and XRD confirmed the formation of uniform fibers with nanodiameters and the presence of Cu nanoparticles onto the electrospun nanofibers. The antibacterial activity of copper was observed against all of the selected bacteria, but the Gram-positive bacteria were more sensitive compared to Gram-negative bacteria. CONCLUSION: According to the obtained results, the hydrogel wound dressing prepared in this research can be effective in caring for open wounds in the early stages of wound healing and preventing the occurrence of prolonged open wounds. Dove 2021-01-11 /pmc/articles/PMC7810733/ /pubmed/33469282 http://dx.doi.org/10.2147/IJN.S266692 Text en © 2021 Ghasemian Lemraski et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Ghasemian Lemraski, Ensieh Jahangirian, Hossein Dashti, Maryam Khajehali, Elaheh Sharafinia, Soheila Rafiee-Moghaddam, Roshanak Webster, Thomas J Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment |
title | Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment |
title_full | Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment |
title_fullStr | Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment |
title_full_unstemmed | Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment |
title_short | Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment |
title_sort | antimicrobial double-layer wound dressing based on chitosan/polyvinyl alcohol/copper: in vitro and in vivo assessment |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810733/ https://www.ncbi.nlm.nih.gov/pubmed/33469282 http://dx.doi.org/10.2147/IJN.S266692 |
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