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Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma

Increasing evidence has revealed that cancer cells undergoing an intermediate state, partial epithelial mesenchymal transition (p‐EMT), tend to metastasize rather than complete EMT. We performed a comprehensive analysis of E‐cadherin and 25 p‐EMT‐related genes in HCC to explore the roles and regulat...

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Autores principales: Lei, Yu, Yan, Wei, Lin, Zhuoying, Liu, Jingmei, Tian, Dean, Han, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810929/
https://www.ncbi.nlm.nih.gov/pubmed/33215860
http://dx.doi.org/10.1111/jcmm.16099
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author Lei, Yu
Yan, Wei
Lin, Zhuoying
Liu, Jingmei
Tian, Dean
Han, Ping
author_facet Lei, Yu
Yan, Wei
Lin, Zhuoying
Liu, Jingmei
Tian, Dean
Han, Ping
author_sort Lei, Yu
collection PubMed
description Increasing evidence has revealed that cancer cells undergoing an intermediate state, partial epithelial mesenchymal transition (p‐EMT), tend to metastasize rather than complete EMT. We performed a comprehensive analysis of E‐cadherin and 25 p‐EMT‐related genes in HCC to explore the roles and regulatory mechanisms of them in HCC. We analysed E‐cadherin and 25 p‐EMT‐related genes in HCC and constructed an mRNA‐miRNA‐lncRNA ceRNA subnetwork containing p‐EMT‐related genes by bioinformatic approaches. IHC was used to identify the protein expression of key p‐EMT‐related genes, P4HA2, ITGA5, MMP9, MT1X and SPP1. Complete EMT is not necessary for HCC progression. Overexpression of P4HA2, ITGA5, MMP9, SPP1 and down‐regulation of MT1X were found in HCC tissues, which were significantly associated with poor prognosis of HCC patients. By means of stepwise reverse prediction and validation from mRNA to lncRNA, an mRNA‐miRNA‐lncRNA ceRNA subnetwork correlated with HCC prognosis was identified by expression and survival analysis. This study implied that key p‐EMT‐related genes P4HA2, ITGA5, MMP9, MT1X, SPP1 could be prognostic biomarkers and potential targets of therapy for HCC patients. We constructed an mRNA‐miRNA‐lncRNA subnetwork containing p‐EMT‐related genes successfully, among which each component might be utilized as a prognostic biomarker of HCC.
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spelling pubmed-78109292021-01-22 Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma Lei, Yu Yan, Wei Lin, Zhuoying Liu, Jingmei Tian, Dean Han, Ping J Cell Mol Med Original Articles Increasing evidence has revealed that cancer cells undergoing an intermediate state, partial epithelial mesenchymal transition (p‐EMT), tend to metastasize rather than complete EMT. We performed a comprehensive analysis of E‐cadherin and 25 p‐EMT‐related genes in HCC to explore the roles and regulatory mechanisms of them in HCC. We analysed E‐cadherin and 25 p‐EMT‐related genes in HCC and constructed an mRNA‐miRNA‐lncRNA ceRNA subnetwork containing p‐EMT‐related genes by bioinformatic approaches. IHC was used to identify the protein expression of key p‐EMT‐related genes, P4HA2, ITGA5, MMP9, MT1X and SPP1. Complete EMT is not necessary for HCC progression. Overexpression of P4HA2, ITGA5, MMP9, SPP1 and down‐regulation of MT1X were found in HCC tissues, which were significantly associated with poor prognosis of HCC patients. By means of stepwise reverse prediction and validation from mRNA to lncRNA, an mRNA‐miRNA‐lncRNA ceRNA subnetwork correlated with HCC prognosis was identified by expression and survival analysis. This study implied that key p‐EMT‐related genes P4HA2, ITGA5, MMP9, MT1X, SPP1 could be prognostic biomarkers and potential targets of therapy for HCC patients. We constructed an mRNA‐miRNA‐lncRNA subnetwork containing p‐EMT‐related genes successfully, among which each component might be utilized as a prognostic biomarker of HCC. John Wiley and Sons Inc. 2020-11-20 2021-01 /pmc/articles/PMC7810929/ /pubmed/33215860 http://dx.doi.org/10.1111/jcmm.16099 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Lei, Yu
Yan, Wei
Lin, Zhuoying
Liu, Jingmei
Tian, Dean
Han, Ping
Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma
title Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma
title_full Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma
title_fullStr Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma
title_full_unstemmed Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma
title_short Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma
title_sort comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810929/
https://www.ncbi.nlm.nih.gov/pubmed/33215860
http://dx.doi.org/10.1111/jcmm.16099
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