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Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes
A large proportion of ankle osteoarthritis (OA) has an early onset and is post‐traumatic. Surgical interventions have low patient satisfaction and relatively poor clinical outcome, whereas joint‐preserving treatments, which rely on endogenous multipotential stromal cells (MSCs), result in suboptimal...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810934/ https://www.ncbi.nlm.nih.gov/pubmed/33305883 http://dx.doi.org/10.1111/jcmm.15993 |
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author | Jones, William G. El‐Jawhari, Jehan J. Brockett, C. L. Koria, Lekha Ktistakis, Ioannis Jones, Elena |
author_facet | Jones, William G. El‐Jawhari, Jehan J. Brockett, C. L. Koria, Lekha Ktistakis, Ioannis Jones, Elena |
author_sort | Jones, William G. |
collection | PubMed |
description | A large proportion of ankle osteoarthritis (OA) has an early onset and is post‐traumatic. Surgical interventions have low patient satisfaction and relatively poor clinical outcome, whereas joint‐preserving treatments, which rely on endogenous multipotential stromal cells (MSCs), result in suboptimal repair. This study investigates MSC presence and potency in OA‐affected talocrural osteochondral tissue. Bone volume fraction (BV/TV) changes for the loading region trabecular volume and subchondral bone plate (SBP) thickness in OA compared with healthy tissue were investigated using microcomputed tomography. CD271‐positive MSC topography was related to bone and cartilage damage in OA tissue, and in vitro MSC potency was compared with control healthy iliac crest (IC) MSCs. A 1.3‐ to 2.5‐fold SBP thickening was found in both OA talus and tibia, whereas BV/TV changes were depth‐dependent. MSCs were abundant in OA talus and tibia, with similar colony characteristics. Tibial and talar MSCs were tripotential, but talar MSCs had 10‐fold lower adipogenesis and twofold higher chondrogenesis than IC MSCs (P = .01 for both). Cartilage damage in both OA tibia and talus correlated with SBP thickening and CD271+ MSCs was 1.4‐ to twofold more concentrated near the SBP. This work shows multipotential MSCs are present in OA talocrural subchondral bone, with their topography suggesting ongoing involvement in SBP thickening. Potentially, biomechanical stimulation could augment the chondrogenic differentiation of MSCs for joint‐preserving treatments. |
format | Online Article Text |
id | pubmed-7810934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78109342021-01-22 Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes Jones, William G. El‐Jawhari, Jehan J. Brockett, C. L. Koria, Lekha Ktistakis, Ioannis Jones, Elena J Cell Mol Med Original Articles A large proportion of ankle osteoarthritis (OA) has an early onset and is post‐traumatic. Surgical interventions have low patient satisfaction and relatively poor clinical outcome, whereas joint‐preserving treatments, which rely on endogenous multipotential stromal cells (MSCs), result in suboptimal repair. This study investigates MSC presence and potency in OA‐affected talocrural osteochondral tissue. Bone volume fraction (BV/TV) changes for the loading region trabecular volume and subchondral bone plate (SBP) thickness in OA compared with healthy tissue were investigated using microcomputed tomography. CD271‐positive MSC topography was related to bone and cartilage damage in OA tissue, and in vitro MSC potency was compared with control healthy iliac crest (IC) MSCs. A 1.3‐ to 2.5‐fold SBP thickening was found in both OA talus and tibia, whereas BV/TV changes were depth‐dependent. MSCs were abundant in OA talus and tibia, with similar colony characteristics. Tibial and talar MSCs were tripotential, but talar MSCs had 10‐fold lower adipogenesis and twofold higher chondrogenesis than IC MSCs (P = .01 for both). Cartilage damage in both OA tibia and talus correlated with SBP thickening and CD271+ MSCs was 1.4‐ to twofold more concentrated near the SBP. This work shows multipotential MSCs are present in OA talocrural subchondral bone, with their topography suggesting ongoing involvement in SBP thickening. Potentially, biomechanical stimulation could augment the chondrogenic differentiation of MSCs for joint‐preserving treatments. John Wiley and Sons Inc. 2020-12-11 2021-01 /pmc/articles/PMC7810934/ /pubmed/33305883 http://dx.doi.org/10.1111/jcmm.15993 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Jones, William G. El‐Jawhari, Jehan J. Brockett, C. L. Koria, Lekha Ktistakis, Ioannis Jones, Elena Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes |
title | Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes |
title_full | Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes |
title_fullStr | Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes |
title_full_unstemmed | Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes |
title_short | Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes |
title_sort | multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – presence, potency and relationships to subchondral bone changes |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810934/ https://www.ncbi.nlm.nih.gov/pubmed/33305883 http://dx.doi.org/10.1111/jcmm.15993 |
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