Cargando…

Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro

STRA8 (Stimulated by Retinoic Acid Gene 8) controls the crucial decision of germ cells to engage meiotic division up and down‐regulating genes involved in the meiotic programme. It has been proven as an amplifier of genes involved in cell cycle control and chromosome events, however, how STRA8 funct...

Descripción completa

Detalles Bibliográficos
Autores principales: Desimio, Maria Giovanna, Cesari, Eleonora, Sorrenti, Maria, De Felici, Massimo, Farini, Donatella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810945/
https://www.ncbi.nlm.nih.gov/pubmed/33236849
http://dx.doi.org/10.1111/jcmm.16087
_version_ 1783637404722135040
author Desimio, Maria Giovanna
Cesari, Eleonora
Sorrenti, Maria
De Felici, Massimo
Farini, Donatella
author_facet Desimio, Maria Giovanna
Cesari, Eleonora
Sorrenti, Maria
De Felici, Massimo
Farini, Donatella
author_sort Desimio, Maria Giovanna
collection PubMed
description STRA8 (Stimulated by Retinoic Acid Gene 8) controls the crucial decision of germ cells to engage meiotic division up and down‐regulating genes involved in the meiotic programme. It has been proven as an amplifier of genes involved in cell cycle control and chromosome events, however, how STRA8 functions as negative regulator are not well understood. In this study, we demonstrate that STRA8 can interact with itself and with other basic Helix‐Loop‐Helix (bHLH) transcription factors through its HLH domain and that this domain is important for its ability to negatively interfere with the Ebox‐mediated transcriptional activity of bHLH transcription factors. Significantly, we show that STRA8 interacts with TCF3/E47, a class I bHLH transcription factors, and with SOHLH1, a gonadal‐specific bHLH, in male germ cells obtained from prepuberal mouse testis. We demonstrated that STRA8, indirectly, is able to exert a negative control on the SOHLH1‐dependent stimulation of c‐KIT expression in late differentiating spermatogonia and preleptotene spermatocytes. Although part of this results were obtained only ‘in vitro’, they support the notion that STRA8 interacting with different transcription factors, besides its established role as ‘amplifier’ of meiotic programme, is able to finely modulate the balance between spermatogonia proliferation, differentiation and acquisition of meiotic competence.
format Online
Article
Text
id pubmed-7810945
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-78109452021-01-22 Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro Desimio, Maria Giovanna Cesari, Eleonora Sorrenti, Maria De Felici, Massimo Farini, Donatella J Cell Mol Med Original Articles STRA8 (Stimulated by Retinoic Acid Gene 8) controls the crucial decision of germ cells to engage meiotic division up and down‐regulating genes involved in the meiotic programme. It has been proven as an amplifier of genes involved in cell cycle control and chromosome events, however, how STRA8 functions as negative regulator are not well understood. In this study, we demonstrate that STRA8 can interact with itself and with other basic Helix‐Loop‐Helix (bHLH) transcription factors through its HLH domain and that this domain is important for its ability to negatively interfere with the Ebox‐mediated transcriptional activity of bHLH transcription factors. Significantly, we show that STRA8 interacts with TCF3/E47, a class I bHLH transcription factors, and with SOHLH1, a gonadal‐specific bHLH, in male germ cells obtained from prepuberal mouse testis. We demonstrated that STRA8, indirectly, is able to exert a negative control on the SOHLH1‐dependent stimulation of c‐KIT expression in late differentiating spermatogonia and preleptotene spermatocytes. Although part of this results were obtained only ‘in vitro’, they support the notion that STRA8 interacting with different transcription factors, besides its established role as ‘amplifier’ of meiotic programme, is able to finely modulate the balance between spermatogonia proliferation, differentiation and acquisition of meiotic competence. John Wiley and Sons Inc. 2020-11-25 2021-01 /pmc/articles/PMC7810945/ /pubmed/33236849 http://dx.doi.org/10.1111/jcmm.16087 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Desimio, Maria Giovanna
Cesari, Eleonora
Sorrenti, Maria
De Felici, Massimo
Farini, Donatella
Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro
title Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro
title_full Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro
title_fullStr Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro
title_full_unstemmed Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro
title_short Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro
title_sort stimulated by retinoic acid gene 8 (stra8) interacts with the germ cell specific bhlh factor sohlh1 and represses c‐kit expression in vitro
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810945/
https://www.ncbi.nlm.nih.gov/pubmed/33236849
http://dx.doi.org/10.1111/jcmm.16087
work_keys_str_mv AT desimiomariagiovanna stimulatedbyretinoicacidgene8stra8interactswiththegermcellspecificbhlhfactorsohlh1andrepressesckitexpressioninvitro
AT cesarieleonora stimulatedbyretinoicacidgene8stra8interactswiththegermcellspecificbhlhfactorsohlh1andrepressesckitexpressioninvitro
AT sorrentimaria stimulatedbyretinoicacidgene8stra8interactswiththegermcellspecificbhlhfactorsohlh1andrepressesckitexpressioninvitro
AT defelicimassimo stimulatedbyretinoicacidgene8stra8interactswiththegermcellspecificbhlhfactorsohlh1andrepressesckitexpressioninvitro
AT farinidonatella stimulatedbyretinoicacidgene8stra8interactswiththegermcellspecificbhlhfactorsohlh1andrepressesckitexpressioninvitro