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Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction

Chronic intermittent hypoxia (CIH) is the primary feature of obstructive sleep apnoea (OSA), a crucial risk factor for cardiovascular diseases. Long non‐coding RNAs (lncRNAs) in myocardial infarction (MI) pathogenesis have drawn considerable attention. However, whether CIH participates in the modula...

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Autores principales: Hu, Chaowei, Li, Jing, Du, Yunhui, Li, Juan, Yang, Yunyun, Jia, Yifan, Peng, Lu, Qin, Yanwen, Wei, Yongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810970/
https://www.ncbi.nlm.nih.gov/pubmed/33215878
http://dx.doi.org/10.1111/jcmm.16097
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author Hu, Chaowei
Li, Jing
Du, Yunhui
Li, Juan
Yang, Yunyun
Jia, Yifan
Peng, Lu
Qin, Yanwen
Wei, Yongxiang
author_facet Hu, Chaowei
Li, Jing
Du, Yunhui
Li, Juan
Yang, Yunyun
Jia, Yifan
Peng, Lu
Qin, Yanwen
Wei, Yongxiang
author_sort Hu, Chaowei
collection PubMed
description Chronic intermittent hypoxia (CIH) is the primary feature of obstructive sleep apnoea (OSA), a crucial risk factor for cardiovascular diseases. Long non‐coding RNAs (lncRNAs) in myocardial infarction (MI) pathogenesis have drawn considerable attention. However, whether CIH participates in the modulation of lncRNA profiles during MI is yet unclear. To investigate the influence of CIH on MI, cardiac damage was assessed by histology and echocardiography, and lncRNA and mRNA integrated microarrays were screened. MI mouse model showed myocardial hypertrophy, aggravated inflammation and fibrosis, and compromised left ventricle function under CIH. Compared with normoxia, 644 lncRNAs and 1084 differentially expressed mRNAs were identified following CIH for 4 weeks, whereas 1482 lncRNAs and 990 mRNAs were altered at 8 weeks. Strikingly, reoxygenation after CIH markedly affected 1759 lncRNAs and 778 mRNAs. Of these, 11 lncRNAs modulated by CIH were restored after reoxygenation and were validated by qPCR. The GO terms and KEGG pathways of genes varied significantly by CIH. lncRNA‐mRNA correlation further showed that lncRNAs, NONMMUT032513 and NONMMUT074571 were positively correlated with ZEB1 and negatively correlated with Cmbl. The current results demonstrated a causal correlation between CIH and lncRNA alternations during MI, suggesting that lncRNAs might be responsible for MI aggravation under CIH.
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spelling pubmed-78109702021-01-22 Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction Hu, Chaowei Li, Jing Du, Yunhui Li, Juan Yang, Yunyun Jia, Yifan Peng, Lu Qin, Yanwen Wei, Yongxiang J Cell Mol Med Original Articles Chronic intermittent hypoxia (CIH) is the primary feature of obstructive sleep apnoea (OSA), a crucial risk factor for cardiovascular diseases. Long non‐coding RNAs (lncRNAs) in myocardial infarction (MI) pathogenesis have drawn considerable attention. However, whether CIH participates in the modulation of lncRNA profiles during MI is yet unclear. To investigate the influence of CIH on MI, cardiac damage was assessed by histology and echocardiography, and lncRNA and mRNA integrated microarrays were screened. MI mouse model showed myocardial hypertrophy, aggravated inflammation and fibrosis, and compromised left ventricle function under CIH. Compared with normoxia, 644 lncRNAs and 1084 differentially expressed mRNAs were identified following CIH for 4 weeks, whereas 1482 lncRNAs and 990 mRNAs were altered at 8 weeks. Strikingly, reoxygenation after CIH markedly affected 1759 lncRNAs and 778 mRNAs. Of these, 11 lncRNAs modulated by CIH were restored after reoxygenation and were validated by qPCR. The GO terms and KEGG pathways of genes varied significantly by CIH. lncRNA‐mRNA correlation further showed that lncRNAs, NONMMUT032513 and NONMMUT074571 were positively correlated with ZEB1 and negatively correlated with Cmbl. The current results demonstrated a causal correlation between CIH and lncRNA alternations during MI, suggesting that lncRNAs might be responsible for MI aggravation under CIH. John Wiley and Sons Inc. 2020-11-20 2021-01 /pmc/articles/PMC7810970/ /pubmed/33215878 http://dx.doi.org/10.1111/jcmm.16097 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Hu, Chaowei
Li, Jing
Du, Yunhui
Li, Juan
Yang, Yunyun
Jia, Yifan
Peng, Lu
Qin, Yanwen
Wei, Yongxiang
Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction
title Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction
title_full Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction
title_fullStr Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction
title_full_unstemmed Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction
title_short Impact of chronic intermittent hypoxia on the long non‐coding RNA and mRNA expression profiles in myocardial infarction
title_sort impact of chronic intermittent hypoxia on the long non‐coding rna and mrna expression profiles in myocardial infarction
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810970/
https://www.ncbi.nlm.nih.gov/pubmed/33215878
http://dx.doi.org/10.1111/jcmm.16097
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