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Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of Caenorhabditis elegans
Standard laboratory culture of Caenorhabditis elegans utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for p...
Autores principales: | Hibshman, Jonathan D., Webster, Amy K., Baugh, L. Ryan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811050/ https://www.ncbi.nlm.nih.gov/pubmed/33490989 http://dx.doi.org/10.1016/j.xpro.2020.100276 |
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