A thermogenetics protocol for detecting gap junction channels in Drosophila egg chambers

In this protocol, we took a “thermogenetics” approach to detect functional gap junction channels between cells in Drosophila egg chambers. We expressed the thermosensitive cation channel TrpA1-A in the germline using Gal4, and a fluorescent [Ca(2+)] sensor (GCaMP6), in all follicle cells using the L...

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Detalles Bibliográficos
Autores principales: Miao, Guangxia, Montell, Denise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811055/
https://www.ncbi.nlm.nih.gov/pubmed/33490985
http://dx.doi.org/10.1016/j.xpro.2020.100269

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811055/
https://www.ncbi.nlm.nih.gov/pubmed/33490985
http://dx.doi.org/10.1016/j.xpro.2020.100269

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