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A thermogenetics protocol for detecting gap junction channels in Drosophila egg chambers
In this protocol, we took a “thermogenetics” approach to detect functional gap junction channels between cells in Drosophila egg chambers. We expressed the thermosensitive cation channel TrpA1-A in the germline using Gal4, and a fluorescent [Ca(2+)] sensor (GCaMP6), in all follicle cells using the L...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811055/ https://www.ncbi.nlm.nih.gov/pubmed/33490985 http://dx.doi.org/10.1016/j.xpro.2020.100269 |