A protocol for rapid pericyte differentiation of human induced pluripotent stem cells

Pericytes play a critical role in promoting, regulating, and maintaining numerous vascular functions. Their dysfunction is a major contributor to the progression of vascular and neurodegenerative diseases, making them an ideal candidate for large-scale production for disease modeling and regenerativ...

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Detalles Bibliográficos
Autores principales: Aisenbrey, Elizabeth A., Torr, Elizabeth, Johnson, Hunter, Soref, Cheryl, Daly, William, Murphy, William L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811164/
https://www.ncbi.nlm.nih.gov/pubmed/33490977
http://dx.doi.org/10.1016/j.xpro.2020.100261
Descripción
Sumario:Pericytes play a critical role in promoting, regulating, and maintaining numerous vascular functions. Their dysfunction is a major contributor to the progression of vascular and neurodegenerative diseases, making them an ideal candidate for large-scale production for disease modeling and regenerative cell therapy. This protocol describes the rapid and robust differentiation of pericytes from human induced pluripotent stem cells (hiPSCs) while simultaneously generating a population of hiPSC-derived endothelial progenitor cells. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2017).

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