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FACS protocol for direct comparison of cell populations isolated from mice

A FACS protocol is described that eliminates isolation and staining artifacts to allow accurate comparison between cell populations isolated from organs obtained from disparate mouse groups. This protocol was validated by characterizing the estrogen receptor positive cells within the mammary gland o...

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Detalles Bibliográficos
Autores principales: Ludwik, Katarzyna A., Sandusky, Zachary M., Wright, Eric B., Lannigan, Deborah A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811174/
https://www.ncbi.nlm.nih.gov/pubmed/33490986
http://dx.doi.org/10.1016/j.xpro.2020.100270
Descripción
Sumario:A FACS protocol is described that eliminates isolation and staining artifacts to allow accurate comparison between cell populations isolated from organs obtained from disparate mouse groups. This protocol was validated by characterizing the estrogen receptor positive cells within the mammary gland of transgenic mice with different genotypes at different stages of the estrous cycle. We include protocols necessary to batch stage animals within the cycle to proceed directly to FACS, which provides optimal RNA yields for RNA-seq. For complete details on the use and execution of this protocol, please refer to Ludwik et al. (2020).